裸小鼠胃癌原位移植模型的建立  

Establishment of an orthotopic gastric cancer model in nude mice

作　　者：田树红[1] 王日超 肖敏[1] 符健[1] TIAN Shu-hong WANG Ri-chao XIAO Min FU Jian(Hainan Research Center for Drug Safety Evaluation, Hainan Medical College, Haikou 571199, China)

机构地区：[1]海南医学院海南省药物安全性评价研究中心,海南海口571199

出　　处：《中国比较医学杂志》2017年第2期21-24,共4页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金资助项目(NO 81160408)

摘　　要：目的建立一种新的裸小鼠胃癌原位移植模型。方法采用Matrx VIP 3000型气体麻醉系统对实验动物进行麻醉。分别用"包埋法"、"挂线法"、和"胃囊法"建立BALB/c裸小鼠胃癌原位移植瘤模型。术后用Caliper IVIS Kinetic小动物活体成像系统对实体瘤的生长情况进行检测和分析。术后28 d对肿瘤组织进行组织病理学检查。结果采用包埋法建立胃癌原位移植裸小鼠模型,除了与其它常用方法一样具有高移植成功率以外,还具有操作简便、时间短、难度低、肿瘤组织不易与其它组织直接接触等特点。结论包埋法能快速制备大批量小鼠胃癌原位移植模型,为胃癌原位移植模型相关研究提供便利。Objective To establish a new orthotopic gastric cancer model in nude mice. Methods The BALB/c nude mice were anesthesized by Matrx VIP 3000 type gas anesthesia systems. Orthotopic gastric cancer models were established by embedding method,thread-ligation method or gastric bursa method in the nude mice. The tumor growth after transplantation in living body was detected by a Caliper IVIS Kinetic small animal imaging system. Samples of the transplanted tumor tissues for histological examination were taken at 28 days after transplantation. Results The orthotopic gastric cancer model was successfully established by embedding method. Compared with the models prepared by other commonly used methods,it showed obvious advantages such as a high success rate of transplantation,simple operation,short time,less difficulty,and less direct contact of the implanted tumor tissue with other organs,etc. Conclusions Large amount of mouse models of orthotopic gastric cancer can be prepared rapidly by the embedding method,thus,provide a convenient tool for related research of gastric cancer in future.

关 键 词：胃癌 原位移植 小动物活体成像 气体麻醉 

分 类 号：R-33[医药卫生]