氟化物和CPP-ACP促进乳牙早期釉质龋再矿化的体外研究  

Study on remineralization of initial enamel caries on primary teeth promoted by fluoride therapy and CPP-ACP in vitro

作　　者：冀美玲[1] 薛欣[1] 朱颐馨[1] 郭桂莲[2] 刘英群[1] 

机构地区：[1]哈尔滨医科大学口腔医学院儿童口腔科,黑龙江哈尔滨150001 [2]哈尔滨医科大学口腔医学院口腔颌面外科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2016年第6期525-528,共4页Journal of Harbin Medical University

基　　金：黑龙江省教育厅科学技术研究项目(12531323)

摘　　要：目的比较氟化物和酪蛋白磷酸多肽钙磷复合体(CPP-ACP)对乳牙早期釉质龋再矿化的作用。方法选取因滞留需要拔除的健康下颌乳中切牙40颗,制备乳牙早期龋模型后随机分为A、B、C、D四组,分别涂布去离子水、氟化物、CPP-ACP、氟化物+CPP-ACP进行再矿化处理。采用显微硬度测量仪测量处理前后各组试件的表面硬度,扫描电子显微镜观察牙釉质表面形态的变化。结果脱矿前、后各组内试件的显微硬度无明显差别(P>0.05);分组再矿化处理后,B、C、D组试件的显微硬度值明显高于A组(P<0.05),且D组硬度显著高于B、C组(P<0.05)。扫描电镜观察发现,再矿化处理后除对照组试件外,其余3组试件均可见矿物质沉积,D组矿物质沉积更明显。结论氟化物和CPP-ACP均对乳牙早期釉质龋有再矿化作用,两者联合使用时再矿化效果更好。Objective To compare the remineralization of initial enamel caries on primary teeth by fluoride therapy and CPP-ACP. Methods A total of 40 samples of retained mandibular primary central incisors were collected, artificial enamel caries lesions were build and randomly divided into four groups： group A （control group） , group B （fluoride therapy group） , group C （CPP-ACP group） and group D （fluoride therapy ＋ CPP-ACP group）. Remineralized by the deionized water, fluoride therapy, CPP-ACP and fluoride therapy ＋ CPP-ACP. The surface hardness （ mierohardness value） of samples before and after treatment was measured by the microhardness tester. The morphological changes of enamel surface was observed by the scanning electron microscope. Results The differences of microhardness values of four groups before and after demineralization treatment were not statistically significant （P 〉 0.05 ）. After remineralization, the microhardness values of group B, C, D were higher than the group A （P 〈 0. 05 ）, the group D was statistically significant higher than group B and C （ P 〈 0. 05 ）. The mineral deposit was found by the scanning electron microscope in samples of three groups afterremineralization, except for samples of the group A. Conclusion Remineralization agents fluoride therapy, CPP-ACP can obviously promote the remineralization of initial enamel caries on primary teeth, combining application of fluoride therapy and CPP-ACP can strengthen the effects.

关 键 词：氟化物 酪蛋白磷酸多肽钙磷复合体 乳牙 早期龋 再矿化 

分 类 号：R781.1[医药卫生—口腔医学]