谷氨酸转运体和γ-氨基丁酸转运体在脑缺血模型中的表达意义  

Significance of expressions of glutamate transporters and γ-aminobutyric acid transporters in rat model with cerebral ischemia

作　　者：任力 邢进 魏子龙 王之涵 赵亮 邱永明[2] 林盈盈[2] 

机构地区：[1]上海市浦东医院神经外科,上海201399 [2]上海交通大学附属仁济医院神经外科,上海200127

出　　处：《实用临床医药杂志》2017年第3期48-51,共4页Journal of Clinical Medicine in Practice

基　　金：上海市卫生和计划生育委员会面上项目(201540412);上海市卫生和计划生育委员会中医药科研基金课题(2014JP025A);上海市浦东新区卫生系统重点专科建设项目(PWZz2013-18)

摘　　要：目的探讨谷氨酸转运体和γ-氨基丁酸转运体在脑缺血模型中的表达意义。方法在体内缺血、体外缺氧2种模型中利用免疫荧光、Westen-blot、RT-PCR等技术定性定量的检测主要的谷氨酸转运体-1(EAAC-1)和γ-氨基丁酸转运体-1(GAT-1)的表达情况。结果在局灶性脑缺血模型(大鼠脑中动脉缺血模型)和缺氧条件下培养的原代神经元中均发现,EAAC1表达下降,GAT1表达上升。结论在脑缺血缺氧状态下EAAC1表达下降,GAT1表达上调。Objective To explore the significance of expressions of glutamate transporters and γ-aminobutyric acid transporters in the rat model with cerebral ischemia. Methods The detections of the expressions of glutamate transporter-1 （ EAAC-1 ） and γ-aminobutyric acid transporter -1 （GAT-1） in models with ischemia in vivo and hypoxia in vitro were performed by immunofluores- cence, Westen-blot and real-time polymerase chain reaction （RT-PCR） methods. Results The ex- pression of EAAC1 was reduced in the cerebrum of focal cerebral ischemic MACO rat model as well as in primary neurons cultured under hypoxia. The expression of GAT1 was elevated. Conclusion EAAC1 expression decreases while GAT1 expression increases under condition of cerebral ischemia and anoxia.

关 键 词：脑缺血 缺氧 谷氨酸转运体1 γ-氨基丁酸转运体1 

分 类 号：R743[医药卫生—神经病学与精神病学]