二氯乙酸钠对人骨肉瘤MG63细胞周期及凋亡影响的初步研究  

Effect of Sodium Dichloroacetate on Cell Cycle and Apoptosis of Human Osteosarcoma Cell Line MG63

作　　者：于雅丽[1] 王雷鸣[1] YU Yali WANG Leiming(Department of Clinical Laboratory, Zhengzhou City Osteopathic Hospital, Zhengzhou 450052, Chin)

机构地区：[1]郑州市骨科医院检验科,河南郑州450052

出　　处：《肿瘤基础与临床》2017年第1期7-10,共4页journal of basic and clinical oncology

基　　金：郑州市科技计划项目(编号:153PKJGG084)

摘　　要：目的探讨不同浓度的二氯乙酸钠(DCA)对人骨肉瘤MG63细胞活性、周期及凋亡的影响。方法用含质量分数10%胎牛血清的RPMI-1640培养基培养MG63细胞,不同浓度DCA作用于对数生长期的细胞,CCK8试剂盒检测50、100、200μg·mL^(-1)DCA处理24 h、48 h后细胞增殖情况;流式细胞术检测100、200μg·mL^(-1)DCA-Na作用24 h、48 h后细胞周期和凋亡情况;qRT-PCR检测100、200μg·mL^(-1)DCA处理后细胞周期相关基因CDK2的变化。结果 CCK8检测结果显示50、100、200μg·mL^(-1)DCA均能抑制MG63细胞的生长,且有时间和浓度依赖性;不同浓度的DCA-Na分别干预MG63细胞不同时间后都明显阻滞细胞于G2/M期,且细胞凋亡率增加(P均<0.001);不同浓度的DCA干预MG63细胞后,细胞中的CDK2mRNA的相对表达量明显降低(P均<0.001)。结论 DCA通过抑制CDK2使MG63细胞阻滞于G2/M期,进而诱导细胞凋亡,抑制细胞的生长。Objective To determine the effect of sodium dichloroacetate （DCA） on human osteosarcoma cell line MC,63 in vitro activity, cell cycle and apoptosis. Methods The MG63 cells were cultured with RPMI-1640 medium including 10% of fetal bovine serum as usual, and treated with different concentration DCA. Then CCK8 kit was used to observe growth suppression of cells under different concentrations of DCA at 24 h ,48 h ; Flow cytometry assay was used to determine the cycle and apoptosis of the cells that were treated by different concentrations of DCA for 24 h ,48 h ,respectively. Finally, qRT-PCR was used to determine the mRNA relative expression of cell cycle related gene CDK2 after DCA invention. Results Different concentration of DCA inhibited cell growth and proliferation in a time-and dosedependent manner; different concentration of DCA-Na acting on different time respectively arrested cell cycle G2/M phase, and improved apoptosis（ P 〈 0. 001 ） ; and decreased the expression of CDK2 gene（ P 〈 0. 001 ）. Conclusion DCA can arrest G2/M cell cycle phase through inhibiting activity of CDK2, and then DCA induce apoptosis of MG63 cells and inhibit the growth of tumor cells.

关 键 词：二氯乙酸钠 骨肉瘤 周期蛋白依赖激酶 细胞周期 

分 类 号：R738.1[医药卫生—肿瘤]