不同治疗效果的艾滋病患者抗病毒治疗前HIV-1 gp120准种特征差异  

Analysis of quasispecies characteristics in HIV-1 gpl20 from AIDS patients with different treatment outcomes

作　　者：欧阳雅博[1] 孔德生[2] 王彦[2] 王琛[2] 李臻鹏[2] 焦洋[2] 殷倩倩[2] 陈德喜[1] 马丽英[2] OU YANG Ya-bo KONG De-sheng WANG Yan WANG Chen LI Zhen-peng JIAO Yang YIN Qian-qian CHEN De-xi MA Li-ying(You ＇an Hospital Affiliated to Capital Medical University, Beijing 100069, China)

机构地区：[1]首都医科大学附属北京佑安医院北京市肝病研究所,北京100069 [2]中国疾病预防控制中心性病艾滋病预防控制中心

出　　处：《国际病毒学杂志》2017年第1期17-21,共5页International Journal of Virology

基　　金：国家自然科学基金（81601796）;首都卫生发展科研专项（2014-1-1151）;北京市优秀人才培养资助青年骨干个人项目（2015000021469G183）;佑安肝病艾滋病基金资助中青年人才孵育项目（YNKT20160015）;北京市属医学科研院所公益发展改革试点项目（京医研2016-2）

摘　　要：目的探讨HIV-1 gpl20准种在不同治疗效果的艾滋病患者抗病毒治疗前的特征差异。方法回顾性收集治疗方案为AZT＋NVP＋3TC的艾滋病患者在抗病毒治疗前的血浆样本，包括病毒抑制（vs）组12例，治疗失败（TF）组12例。采用单基因组扩增技术获得gp120准种序列，分析比较遗传多样性、氨基酸长度、潜在糖基化位点及特征性氨基酸的特点。结果本研究共获得gpl20序列365条序列，其中vs组168条（6-20条），TF组197条（7-28条）。TF组的gpl20准种复杂度高于vs组，差异有统计学意义（P=O．003）；TF组的gpl20准种ds及dN中位数均高于vs组（P=0．017；P=0．002）。TF组HIV-1准种gpl20氨基酸长度长于VS组（P=0．001）。TF与vs组的gpl20准种相比共有9个氨基酸位点存在明显差异，多数分布在V1/V2区（6／9，66．6％）。结论不同治疗效果的艾滋病患者治疗前的HIV-1gpl20准种基因特征存在差异，TF组患者来源的HIV-1准种遗传多样性更高。Objective To investigate patients with different treatment outcomes quasispecies characteristics of HIV-I gpl20 from AIDS Methods Plasma samples of AIDS patients received AZT＋NVP＋3TC regimens were retrospectively collected, including 12 virologic suppression （VS） patients and 12 treatment failure （TF） patients. HIV-1 gp 120 quasispecies sequences by single genome amplification （SGA） methods were collected to study the characteristics and differences of quasispecies diversity, dS and dN, amino acid length, numbers of potential N-linked glycosylation sites （PNGS） and signature pattern between the two groups. Results A total of 365 gp 120 sequences were obtained, including 168 sequences （6-20） in VS group and 197 sequences （7-28） in TF groups. A higher diversity was noted for the variants in TF group than that VS group （P = 0.003）. Median dS and dN were higher in TF group （P = 0.017, P = 0.002）. A longer viral peptide in TF group was found （P = 0.001）. Nine genetic signatures of gp 120 were identified in TF patients, and most of them belonged to V1/V2 region （6/9, 66.6%）. Conclusions Quasispecies genetic characteristics are found between the two groups with different treatment outcome before antiviral therapy, which exhibit high quasispecies diversity in TF-derived strains.

关 键 词：Ⅰ型人类免疫缺陷病毒 GP120 准种 抗病毒治疗 治疗效果 

分 类 号：R512.91[医药卫生—内科学]