TGIF2调控胶质瘤细胞的增殖和迁移  

TGIF2 regulates the proliferation and migration of glioma cells

作　　者：杨亚丽[1] 宋超[1] 符辉[1] 

机构地区：[1]武汉大学基础医学院人体解剖与组织胚胎学系,武汉430071

出　　处：《中国组织化学与细胞化学杂志》2017年第1期1-6,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金面上项目(81371338);武汉大学自主科研项目(2042016kf0181)

摘　　要：目的探讨TGIF2在胶质瘤中的表达及其对A172胶质瘤细胞增殖和迁移的影响。方法原位杂交技术检测TGIF2 mRNA在小鼠胚胎不同时间点脊髓中的表达;用GSE16011数据库和中国人临床样本检测TGIF2在胶质瘤样本和非癌脑组织中的表达水平;利用shRNA技术下调A172胶质瘤细胞TGIF2的表达后,分别通过CCK-8分析、划痕试验、凋亡蛋白检测研究TGIF2对胶质瘤细胞增殖、迁移和凋亡的影响。结果原位杂交显示,TGIF2 mRNA特异性表达在小鼠E11.5和E13.5脊髓的室管膜区域;TGIF2 mRNA在胶质瘤样本中的表达显著高于非癌脑组织;沉默TGIF-2的表达能显著抑制A172细胞的增殖和迁移能力,促进细胞凋亡。结论 TGIF2mRNA在胶质瘤中高表达;TGIF2可以增强细胞的增殖和迁移能力,抑制细胞凋亡,可能是脑癌诊疗的一个潜在的靶点基因。Objective To investigate the expression of TGIF2 in glioma and its role in the proliferation and migration of A172 glioma cells. Methods The expression pattern of TGIF2 mRNA in the spinal cords of embryonic mice at different developmental stages was detected by in site hybridization. The level of TGIF2 mRNA in glioma tissue and nonneoplastic brain tissue was analyzed in samples from GSE16011 database and the Chinese population. The expression of TGIF2 in A172 glioma cells was blocked by ShRNA, after which the rates of cell proliferation, migration and apoptosis were measured by CCK-8 assay, scratch test and apoptotic study, respec- tively. Results TGIF2 mRNA was specifically expressed in the ventricular zone of mouse spinal cords by in site hybridization at E11.5 （ embryonic day 11.5） and El3.5. The level of TGIF2 mRNA was much higher in glioma than in nonneoplastic brain tissue. TGIF2 si- lencing significantly suppressed the proliferation and migration of A172 glioma cells and induced their apoptosis. Conclusion The ex- pression of TGIF2 mRNA is up-regulated in glioma. TGIF2 can promote the proliferation and migration of glioma cells and suppress their apoptosis, might be a potential target for the diagnosis and treatment of brain tumor.

关 键 词：胶质瘤 TGIF2 增殖 迁移 凋亡 

分 类 号：R73-37[医药卫生—肿瘤]