氧化槐果碱对小鼠炎症性疼痛的镇痛作用研究  

Oxysophocarpine ameliorates carrageenan-induced inflammatory pain via inhibiting expressions of prostaglandin E_2 and cytokines in mice

作　　者：吴璟[1] 杨文[1] 郑婕[2] 杨洋[2] 黎伟华[1] 赵玉萍[2] 李凤梅[2] 马鹏生[2] 余建强[2] 

机构地区：[1]宁夏医科大学实验动物中心,银川750004 [2]宁夏医科大学药学院,银川750004

出　　处：《中国比较医学杂志》2017年第1期54-58,共5页Chinese Journal of Comparative Medicine

基　　金：宁夏高等学校科学研究项目(NGY2014097)

摘　　要：目的研究氧化槐果碱对二甲苯和角叉菜胶诱导的小鼠炎症性疼痛的影响,以探索其可能的机制。方法 ICR小鼠50只,随机分为模型组、ASP治疗组、氧化槐果碱高、中、低剂量组,各组分别给予对应药物1 h后,采用二甲苯致小鼠耳肿胀实验和角叉菜胶诱导的小鼠足趾肿胀实验,研究氧化槐果碱对小鼠各种炎症性刺激的影响。结果与生理盐水组相比,氧化槐果碱各剂量组能明显抑制二甲苯致小鼠耳肿胀程度和角叉菜胶致小鼠足趾肿胀程度;小鼠足组织的TNF-α,IL-1β,IL-6和PGE2水平显著降低。结论 OSC对角叉菜胶诱导的炎性疼痛具有明显的镇痛作用,可能与其抑制炎性因子的分泌有关。Objective In this study we investigated the analgesic effect of oxysophocarpine （OSC） on carrageenan-induced inflammatory pain in mice and to explore its possible mechanism. Methods Mouse ear swelling test and carrageenan-induced paw edema were used to investigate the effects of OSC （10, 40 and 80 mg/kg） on inflammatory pain in mice. The mechanical hyperalgesia and allodynia were measured by yon Frey filaments. Paw edema was assessed by toe volumetric measuring instrument, text the content of TNF-c~, IL-113, IL-6 and PGE2. Results OSC showed a significant anti-inflammatory effect in the mouse ear swelling test. OSC （10, 40 and 80 mg/kg） also significantly reduced the paw edema volume and improved mechanical allodynia threshold value on carrageenan-induced inflammatory pain, as well as relieved paw tissues inflammatory damage and reduced the numbers of neutrophils in mice. OSC significantly suppressed over-expression of TNF-α, IL-1β, IL-6 and PGE2. Conclusions Based on these findings we propose that OSC attenuates inflammatory pain by suppressing the Pro-inflammatory cytokines.

关 键 词：氧化槐果碱 炎性疼痛 炎性细胞因子 

分 类 号：R-332[医药卫生]