烤烟部位间叶面微观形态特征的差异及其与部分外观、物理指标的关系  

Surface microscopic morphology characteristics of flue-cured tobacco from different stalk positions and their relationships with selected apparent and physical quality indexes

作　　者：过伟民[1] 尹启生[1] 张艳玲[1] 何斌 王信民[1] 王广山[1] 

机构地区：[1]中国烟草总公司郑州烟草研究院,河南郑州市高新区枫杨2号450001 [2]湖南省烟草公司郴州市公司,湖南郴州市北湖区423000

出　　处：《中国烟草学报》2017年第1期62-68,共7页Acta Tabacaria Sinica

基　　金：国家烟草专卖局科技项目"蛋白质和游离氨基酸在烟叶质量评价中的应用及主要影响因素研究"(122014AA0610);郑州烟草研究院院长基金项目"基于扫描电镜图像分析的烤烟烟叶微观结构特征研究"(112012CA0060)

摘　　要：为探索微观形态指标在烟叶品质评价中的应用,采用扫描电镜结合图像分析的方法,研究了不同部位烟叶微观形态的差异及与部分外观、物理指标的关系。结果表明:1烤后烟叶表面细胞轮廓清晰,中部向上凸起形成褶皱,随部位上升细胞褶皱呈减弱趋势;2不同部位烟叶表面微观形态差异明显,下部叶表面细胞相对较大且形状不规则,中部叶表面细胞相对较小且形状不规则,上部叶表面细胞形状相对规则;3不同叶片结构和厚度烟叶的细胞形状存在差异,叶片结构相对疏松和厚度相对较薄烟叶的细胞形状相对不规则。4细胞形状的不规则程度对烟叶叶片结构疏松程度存在直接和间接(通过细胞周长)正向作用。烟叶表面细胞形状因子(表征细胞形状不规则程度)在量化评价叶片结构、成熟度等方面有较好应用前景。In order to study the effect of micro morphological characteristics in quality evaluation,flue-cured tobacco leaves of different stalk positions were prepared and investigation was made into differences of microscopic morphology and its relationship with apparentquality and physical index based on SEM combined with image analysis.Results showed that：（1） Cell outline of flue-cured leaves was clear with central part bulged and wrinkled,and the wrinkle declined with the rising of stalk position.（2） Micro-morphological characteristics of different leaf positions showed obvious difference.Lower position leaves had large cell area and irregular cell shapes,while middle position leaves had relatively small cell area and irregular cell shape,and upper position leaves had regular cell shape.（3） Cell shape showed difference among leaves of different leaf structure and thickness,in which cell shape was relatively irregular in leaves with relatively loose structure and thin thickness.（4） The degree of irregularity of cell shape had a direct and indirect（by cell circumference） positive effect on leaf structure.The cell shape factor which characterized the degree of irregularity of cell shape had a better application prospect for evaluating leaf structure and maturity of flue-cured tobacco leaves.

关 键 词：烤烟 表面微观形态 扫描电镜 外观品质 物理特性 

分 类 号：S572[农业科学—烟草工业]