β-榄香烯诱导人黑色素瘤A375细胞凋亡的研究  

Effect of β-elemene on Apoptosis in Human Melanoma A375 Cells

作　　者：蒋革[1] 吴艳华[1] 罗锋[1] 薛勇[1] 李清[2] 

机构地区：[1]大连大学生命科学与技术学院,中国辽宁大连116622 [2]大连大学教务处,中国辽宁大连116622

出　　处：《生命科学研究》2017年第1期55-58,94,共5页Life Science Research

基　　金：辽宁省教育厅科学研究一般项目(L2014494)

摘　　要：β-榄香烯是我国自主开发的二类非细胞毒性抗肿瘤药物,具有广谱、高效、低毒等优点,对肝癌、肺癌等多种恶性肿瘤均有较好的治疗效果,但对人皮肤肿瘤的研究尚无报道。基于此,就β-榄香烯对人恶性黑色素瘤A375细胞的抗肿瘤活性展开研究,发现β-榄香烯能够显著抑制A375细胞增殖(P<0.01),并在一定范围内呈浓度和时间依赖性,主要表现为β-榄香烯能够诱导细胞发生凋亡。进一步对相关凋亡蛋白进行Western-blot检测,发现β-榄香烯通过抑制细胞内AKT信号通路途径,调控Bcl-2家族蛋白,降低Bcl-2的表达,显著增加Bad的表达量,并激活部分caspase系列蛋白质的剪切活化,从而抑制A375细胞增殖,诱导细胞凋亡。β-elemene, a self-developed anticancer medicine in China, has an obvious therapeutic effect on liver cancer, lung cancer and other malignant tumors due to low toxicity, high efficacy and wide-spectrum activity, while its role on human skin cancer has not been reported. Here, the anti-tumor activity of β-elemene was investigated on human melanoma cells. It was found that the proliferation of A375 cells was sig- nificantly inhibited by β-elemene （P〈0.01） in a concentration- and time-dependent manner. Furthermore, related apoptosis proteins were detected by Western-blot, which showed that β-elemene inhibited AKT signal pathway to regulate Bcl-2 family proteins. It reduced the expression of Bcl-2, but significantly increased the expression of Bad, and in the meantime partially activated caspase proteins, thereby inhibiting A375 cell proliferation and inducing apoptosis.

关 键 词：Β-榄香烯 抗肿瘤药物 人黑色素瘤细胞 细胞凋亡 信号机制 

分 类 号：Q291[生物学—细胞生物学] R739.5[医药卫生—肿瘤]