Human papillomavirus(HPV) E6/E7 mRNA detection in cervical exfoliated cells:a potential triage for HPV-positive women  

宫颈脱落细胞人乳头瘤病毒(HPV)E6/E7 mRNA检测在HPV阳性女性筛查中的应用（英文）

作　　者：Ye-li YAO Qi-fang TIAN Bei CHENG Yi-fan CHENG Jing YE Wei-guo LU 

机构地区：[1]Department of Gynecologic Oncology, Women 's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年第3期256-262,共7页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the Special Fund for Scientific Research in the Public Interest from the National Health and Family Planning Commission of the People’s Republic of China(No.201402010);the National Key Technology R&D Program of China(No.2016YFC1302900);the Zhejiang Provincial Educational Project(No.Y201328819);the Zhejiang Provincial Medical & Hygienic Science and Technology Project(Nos.2013KYB147 and 2013KYA104);the Zhejiang Provincial Natural Science Foundation(No.LQ14H160007),China

摘　　要：Cytology triage has been generally recommended for human papillomavirus （HPV）-positive women, but is highly dependent on well-trained cytologists. The present study was designed to explore whether HPV E6/E7 mRNA detection in cervical exfoliated cells can be a potential triage for HPV-positive women from a clinic-based population. Both the primary HPV testing and Papanicolaou （Pap） test were performed on all eligible HPV-positive women. HPV E6/E7 mRNA was detected by QuantiVirus HPV E6/E7 mRNA assay in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. The data were assessed by Pearson＇s Chi-squared test and the receiver operating characteristic curve. A total of 404 eligible HPV-positive women were enrolled. Positive rate of E6/E7 mRNA in high-grade squamous intraepithelial lesion （HSIL） cases was higher than that in low-grade squamous intraepithelial lesion （LSIL） or normal cases. There was no statistical difference found between mRNA and cytological testing with sensitivity （89.52% vs. 86.67%, P=0.671）, specificity （48.96% vs. 48.96%, P=1.000）, positive predictive value （39.00% vs. 38.24%, P=1.000）, and negative predictive value （92.76% vs. 90.97%, P=-0.678） for detecting ≥HSIL. HPV E6/E7 mRNA detection in cervical exfoliated cells shows the same performance as Pap triage for HSIL identification for HPV-positive women. Detection of HPV E6/E7 mRNA may be used as a new triage option for HPV-positive women.目的:基于临床样本探讨宫颈脱落细胞HPV E6/E7mR NA检测能否作为HPV阳性女性的筛查手段。创新点:首次比较了宫颈脱落细胞HPV E6/E7 mR NA检测与宫颈脱落细胞学检测对于HPV阳性女性宫颈活检结果的预测价值。方法:所有入组的HPV阳性女性均进行宫颈脱落细胞学及HPV E6/E7 mR NA检测。比较两种方法对于宫颈活检结果的预测价值。结论:对于HPV阳性的女性,高级别鳞状上皮内病变(HSIL)HPV E6/E7 mR NA阳性率高于低级别鳞状上皮内病变(LSIL)及正常病例。HPV E6/E7mR NA与细胞学检测相比,HSIL及以上级别病变的检测敏感性(89.52%vs.86.67%,P=0.671)、特异性(48.96%vs.48.96%,P=1.000)、阳性预测值(39.00%vs.38.24%,P=1.000)及阴性预测值(92.76%vs.90.97%,P=0.678)均无显著差异。HPV E6/E7 mR NA检测或能成为HPV阳性女性的一种新的筛查手段。

关 键 词：Human papillomavirus （HPV） HPV E6/E7 mRNA High-grade squamous intraepithelial lesion （HSIL） 

分 类 号：R737.33[医药卫生—肿瘤]