阿霉素诱导人乳腺癌细胞MCF-7上皮-间质转化的作用  

Effects of adriamycin on inducing epithelial-mesenchymal transition of human breast cancer cell line MCF-7

作　　者：孙玉今[1] 全松石[1] 金恩浩[1] 赵静玉[1] 李良[1] 金承龙[1] SUN Yujin QUAN Songshi JIN Enhao ZHAO Jingyu LI Liang JIN Chengiong(Affiliated Hospital of Yanbian University, Yanji 133000, Jilin , China)

机构地区：[1]延边大学附属医院,吉林延吉133000

出　　处：《延边大学医学学报》2016年第4期247-249,共3页Journal of Medical Science Yanbian University

摘　　要：[目的]探讨阿霉素(ADM)诱导人乳腺癌细胞MCF-7上皮-间质转化的作用.[方法]体外培养人乳腺癌细胞MCF-7,用不同质量浓度ADM处理后观察形态变化,并采用细胞免疫荧光染色法检测E-cadherin和vimentin蛋白水平.[结果]ADM可使MCF-7细胞变长变扁,明显增加MCF-7细胞的vimentin蛋白水平(P<0.05),降低E-cadherin蛋白水平(P<0.05).[结论]ADM可诱导人乳腺癌细胞MCF-7上皮-间质转化.OBJECTIVE To study the effects of adriamycin（ADM）on inducing epithelial-mesenchymal transition of human breast cancer cell line MCF-7.METHODS Human breast cancer cell line MCF-7 was cultured in vitro with different concentration of ADM,and the morphologic changes of the cells were observed.Immunofluorescence staining technique was used to detect the levels of E-cadherin and vimentin.RESULTSADM could make the MCF-7cell to be long and flat.ADM significantly increased the level of vimentin and decreased the level of E-cadherin in the MCF-7cells（P〈0.05）.CONCLUSION ADM could induce epithelial-mesenchymal transition of human breast cancer cell line MCF-7.

关 键 词：乳腺癌 上皮间质转化 阿霉素 

分 类 号：R737.9[医药卫生—肿瘤]