石楠锈孢锈菌重寄生拟盘多毛孢的分离鉴定及抑菌活性  

Isolation,Identification and Antimicrobial Activity of Mycoparasites(Pestalotiopsis)from Aecidium pourthiaea

作　　者：李靖[1] 谢津[1] 李向楠[1] 周稚凡[1] 刘风路[1] 陈玉惠[1] LI Jing XIE Jin LI Xiang-nan ZHOU Zhi-fan LIU Feng-lu CHEN Yu-hui(Life Science College, Southwest Forestry University, Kunming 65022)

机构地区：[1]西南林业大学生命科学学院,昆明650224

出　　处：《生物技术通报》2017年第3期122-127,共6页Biotechnology Bulletin

基　　金：云南省优势特色重点学科生物学一级学科建设项目(50097505);云南省高校林下生物资源保护及利用科技创新团队项目(51400605)

摘　　要：重寄生拟盘多毛孢因其特殊生境,可产生区别于内生或病原拟盘多毛孢的次生代谢产物,是有待开发的真菌资源。为了获得活性较好的石楠锈孢锈菌重寄生菌,采用组织分离法,从患有锈病的球花石楠叶片上分离纯化出3株疑似拟盘多毛孢真菌,它们形态特征差异明显,通过回接实验证实其均为石楠锈孢锈菌的重寄生菌。对3菌株在不同培养基上的培养性状和显微特征作了描述,并对ITS序列进行了扩增和序列分析。形态学和分子鉴定结果证实这3株真菌均为拟盘多毛孢属真菌。抑菌实验结果表明,3株重寄生菌除了对石楠锈孢锈菌的锈孢子有破坏作用外,对其他10种常见植物病原真菌也有一定的抑制作用。Mycoparasite Pestalotiopsis produces the second metabolites different from endophytic or pathogenic Pestalotiopsis, as an important fungal resource, is yet to be developed and used. In order to obtain highly active mycoparasites of Aecidium pourthiaea, tissue separation method was used to isolate the mycoparasites, and 3 strains were isolated from the leaves of Photinia prionophyUa with rust and suspected to be Pestalotiopsis ; moreover, they were confirmed to be mycoparasites of A. pourthiaea via the tie back experiments though their morphologies varied obviously. Cultural characteristics on different medium and microscopic features of these three strains were described ; also ITS sequences were amplified and analyzed. The morphology and molecular identification confirmed that all 3 strains were Pestalotiopsis. The results of anti-fungus experiment showed that these three strains not only destroyed the aecidiospores of A. pourthiaea, but also inhibited the growth of other 10 plant pathogenic fungi.

关 键 词：拟盘多毛孢 重寄生 分离鉴定 抑菌活性 石楠锈孢锈菌 

分 类 号：S763.7[农业科学—森林保护学]