senex基因在急性髓系白血病中的表达及意义  

Expression of senex gene in acute myeloid leukemia and its clinical significance

作　　者：曹艳芸[1] 陶千山[1] 王会平[1] 胡林辉[1] 张家奎[1] 翟志敏[1] 

机构地区：[1]安徽医科大学第二附属医院血液科,安徽医科大学血液病研究中心,合肥230601

出　　处：《安徽医科大学学报》2017年第3期401-405,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81670179、81401293)

摘　　要：目的探讨senex基因在急性髓系白血病(AML)中的表达水平,并探讨其在AML发生发展中的意义。方法应用实时荧光定量PCR检测senex基因在21例初诊AML患者(初诊组)、27例骨髓完全缓解的AML患者(缓解组)、19例骨髓复发的AML患者(复发组)以及12例对照者(对照组)的骨髓单个核细胞中的表达情况,比较4组之间的基因表达水平差异,并分析senex基因表达水平与临床特征和疗效的关联性。结果初诊组senex基因表达水平高于缓解组(4.06±2.72 vs 2.52±1.83,P=0.04)和对照组(4.06±2.72 vs 1.22±0.48,P=0.001),复发组senex基因表达水平高于对照组(3.27±2.54 vs 1.22±0.48,P=0.017),且各亚型之间以AML-M3的senex基因表达水平最高。AML患者senex基因mRNA表达水平与骨髓原始细胞比例存在相关性(rs=0.557,P=0.000)。此外分析AML初诊患者的临床特征显示1次化疗缓解的患者(n=14)的senex基因表达显著低于1次化疗未缓解的患者(n=7)(1.77±1.47 vs 4.85±2.20,P=0.003),并且1次化疗未缓解的患者预后均较差。结论 senex基因的异常表达可能与AML的发生发展以及预后密切相关。Objective To investigate the mRNA expression of senex gene in acute myeloid leukemia（ AML）, and then evaluate the significance in AML development and progression. Methods Real-time quantitative polymerase chain reaction （qRT-PCR） was performed to examine senex mRNA expression in the bone marrow mononuclear cells from 21 newly diagnosed （ND） AML patients, 27 completely remission （CR） AML patients, 19 relapse AML patients, and 12 controls, then the differences in gene expression levels among the four groups were compared, the correlations between senex mRNA, clinical features and efficacy were also analyzed. Results The level of senex mRNA expression in ND group was higher than CR （4.06 ± 2.72 vs 2. 52± 1.83, P 〈 0. 05 ） and normal group （4.06 ±2. 72 vs 1.22 ±0. 48, P 〈0.01 ）. The senex mRNA expression in relapsing group was increased compared with normal group （3.27 ± 2.54 vs i. 22 ± 0. 48, P 〈 0.05 ）, and then the data was showed that the level of senex mRNA in subtype of AML-M3 was highest among all subtypes. The correlation coefficient between the level of senex mRNA expression and blasts of bone marrow in AML patients was remarkable （ rs = 0. 557, P 〈 0. 01 ）. In addi- tion, to analyze the clinical features of patients with ND patients, the data indicated that senex gene expression in AML patients achieved CR（ n = 14） was significantly lower than patients with no CR （ n = 7） （ 1. 77 ± 1.47 vs 4. 85 ±2. 20, P 〈0.01） after first chemotherapy. And the prognosis of these 7 cases with no CR is all poor. Conclu- sion Abnormal senex mRNA expression may be closely correlated to development, progression and prognosis in AML.

关 键 词：急性髓系白血病 senex 细胞衰老 复发 凋亡抵抗 

分 类 号：R733.71[医药卫生—肿瘤]