微囊藻毒素HPLC快速检测方法研究  

Study on Rapid Analysis of Microcystins by HPLC

作　　者：沈强[1] 李嗣新[1] 胡俊[1] SHEN Qiang LI Sixin HU Jun(Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, Chin)

机构地区：[1]水利部中国科学院水工程生态研究所,水利部水工程生态效应与生态修复重点实验室,湖北武汉430079

出　　处：《环境科学与技术》2017年第2期103-106,共4页Environmental Science & Technology

基　　金：水利部948项目(201509);国家自然科学基金项目(51279112)

摘　　要：该研究在传统的微囊藻毒素HPLC分析方法的基础上,通过优化选择色谱柱、流动相和流速的方法,建立了一种快速分析微囊藻毒素的HPLC检测体系。采用该检测体系,微囊藻毒素变体MC-RR、MC-LR的保留时间分别为(2.609±0.007)min和(2.959±0.009)min(n=5),MC-LR、MC-RR在0.4~100μg/m L的浓度范围内线性关系良好。与传统的HPLC检测方法相比,微囊藻毒素分析时间被大大缩短,具较好的应用前景。An improved HPLC system was set up for rapid analysis of Microcystins（MCs）based on the traditional HPLC method. The retention time of MC-RR and MC-LR were shortened to 2.609 ±0.007 min and 2.959 ±0.009 min respectively.However,linear relationships were satisfactory in 0.4 μg/m L -100 μg/m L. This improved HPLC system showed great superiority for analyses of MCs. The analysis time was shortened within 3 min,and the method has good application prospects.

关 键 词：微囊藻毒素 快速检测 HPLC 

分 类 号：X132[环境科学与工程—环境科学]