白芷种子蛋白质提取方法的比较  

Comparison of Protein Extraction Methods for Angelica dahurica Seeds

作　　者：吴萍[1] 郭俊霞[1] 李青苗[1] 夏燕莉[1] 张美[1] 肖特[1] 方清茂[1] WU Ping GUO Jun-xia LI Qing-miao et al(Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu, Sichuan 610041)

机构地区：[1]四川省中医药科学院,四川成都610041

出　　处：《安徽农业科学》2017年第6期118-120,123,共4页Journal of Anhui Agricultural Sciences

基　　金：四川省基本科研业务专项(A2015N13);四川省科技创新苗子工程(2016077)

摘　　要：[目的]建立适用于白芷种子的SDS-PAGE蛋白质样品提取方法。[方法]比较5种提取方法所获得白芷种子总蛋白质产量以及SDS-PAGE电泳的谱带数目、强度和电泳分辨率等方面的差异。[结果]方法 a所提蛋白质含量高,杂质少,电泳谱带清晰;方法 b所提蛋白质溶液电泳谱带清晰,分辨率较高,但提取含量较低;方法 c和d所提蛋白质杂质较多,纯度不高,分辨率较低;方法 e所提蛋白质纯度较高,但蛋白条带缺失较多。[结论]方法 a提取白芷种子蛋白质的效果最好,所提蛋白效率较高,杂质少,电泳谱带清晰,分辨率高,谱带数多,优于其他提取法。[ Objective ] To establish a suitable protein extraction protocol for total proteins extraction from Angelica dahurica seeds. [ Method ] We compared the total protein yield and SDS-PAGE eleetrophoretic band number and strength, leetrophoresis resolution by five different methods. [ Result] Method a had higher protein content, less impurity and clearer electrophoresis band. Method b had clearer eleetrophoresis band, higher resolution, but lower protein content. Methods c and d had more impurity, lower purity and resolution. Method e had higher protein purity, but more lack of protein bands. [ Conclusion] Method a has the best extraction effects of protein from A. dahurica seeds, which has higher protein extraction efficiency, less impurity, clearer band, higher resolution, more band number. Thus, method a is superior to other four methods.

关 键 词：白芷种子 蛋白质提取 聚丙烯酰氨凝胶电泳 

分 类 号：S567.19[农业科学—中草药栽培]