脑出血后氧化应激对神经细胞凋亡及c-myc蛋白表达的影响  

Effects of oxidative stress on neuronal apoptosis and protein expression of c-myc after intracerebral hemor rhage

作　　者：汪立松 章永强 冯忠 应小卫 王希佳 霍鑫龙 

机构地区：[1]温岭市第一人民医院神经内科,浙江温岭317500

出　　处：《中华全科医学》2017年第4期608-610,676,共4页Chinese Journal of General Practice

摘　　要：目的观察脑出血后氧化应激与神经细胞凋亡的相关性及对凋亡相关蛋白c-myc表达的影响。方法选择SD雄性大鼠84只,随机分为假手术组、模型组、MPEG-SOD组,每组28只,自体血注入法建立脑出血模型,MPEG-SOD组建模前腹腔注射MPEG-SOD;术后12 h、1 d、3 d、7 d不同时间点行神经功能缺陷评分(NDS),取脑组织测定脑组织含水量,分光光度法测定丙二醛(MDA)、超氧化物歧化酶(SOD),免疫组化法测定核因子κB(NF-κB)p65阳性细胞表达,TUNEL测定神经细胞凋亡,Western blot测定c-myc蛋白表达水平。结果与假手术组比较,模型组各时点NDS评分、SOD活性明显降低,脑组织含水量、MDA含量、c-myc蛋白、NF-κB p65、TUNEL阳性细胞表达明显升高(P<0.05),术后3 d达峰值,与模型组同时点比较,MPEG-SOD组NDS评分、SOD活性明显升高,脑组织含水量、MDA含量、c-myc蛋白、NF-κB p65、TUNEL阳性细胞表达明显降低(P<0.05),cmyc蛋白表达与TUNEL阳性细胞数呈正相关(模型组r=0.83,MPEG-SOD组r=0.91,P<0.05)。结论脑出血后氧化应激诱导神经细胞凋亡可能与启动凋亡相关基因c-myc有关。Objective To investigate the relationship between oxidative stress and neuronal apoptosis after intracerebral hemorrhage（ ICH）,and the influence of oxidative stress on c-myc expression. Methods Eighty-four male SD rats of 7-week-old were selected and randomly divided into sham operation group,model group,MPEG-SOD group,with 28 rats in each group. Intracerebral hemorrhage model was induced by autologous blood injection. Rats in MPEG-SOD group were injected with MPEG-SOD before modeling. The neurological deficit score（ NDS） was measured at 12 h,1 d,3 d and 7 d after the operation. The water content of brain tissue was measured,and the content of malondialdehyde（ MDA） and superoxide dismutase（ SOD） in brain was measured by spectrophotometric method. The expression of nuclear factor-kappa B（ NF-κB） p65 positive cells was detected by immunohistochemistry. The apoptosis of neurons was measured by TUNEL and the expression of c-myc protein was detected by Western blot. Results Compared with the sham-operated group,the NDS score and SOD activity at each time points were obviously decreased,while the water content in the brain tissue,MDA content,c-myc protein expression,NF-κB p65 and TUNEL-positive cells in the model group were significantly increased（ P〈0. 05）,which reached the peak 3d after the operation. Compared with the model group,the NDS score,SOD activity,water content in the brain tissue,MDA content,c-myc protein expression and TUNEL,NF-κB p65 positive cells in the MPEG-SOD group were significantly decreased（ P〈0. 05）. The expression of c-myc protein and TUNEL-positive cells in the brain tissue was correlated（ R = 0. 83 in model group,as well as r = 0. 91 in MPEG-SOD group,P〈0. 05）. Conclusion The oxidative stress-induced apoptosis of neurons may be related to the initiation of apoptosis-related gene c-myc after intracerebral hemorrhage.

关 键 词：脑出血 神经细胞凋亡 氧化应激 C-MYC蛋白 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R329.25[医药卫生—临床医学]