紫外-可见光分光光度法测定去铁胺的含量  

Determination of deferoxamine content by Ultraviolet-visible spectrophotometry

作　　者：马东来[1,2] 刘爱朋 张园园[1] 马海洋[1] 张建平[1] 楚立[1] 

机构地区：[1]河北中医学院药学院,石家庄050200 [2]河北中医学院中西医结合生殖疾病协同创新中心,石家庄050200

出　　处：《分析试验室》2017年第3期289-291,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(81573669);河北省自然科学基金(H2014206244);河北省科技支撑计划项目(15272506);河北中医学院博士科研基金项目(BSZ2016001)资助

摘　　要：建立了紫外-可见光分光光度法测定去铁胺含量的方法。去铁胺在HAc-NAc缓冲溶液中,与Fe(II)发生显色反应,采用紫外-可见光分光光度法测定其含量。结果表明,去铁胺在0.0897~0.8074 mg/m L范围内,呈现良好的线性关系,回归方程为Y=9.5714X-0.0069(R2=0.9988)。在供试液中分别添加去铁胺对照品0.1,0.2,0.3 mg/m L 3个水平添加下,平均回收率99.5%,相对标准偏差(RSD)为2.0%。方法可用于甲磺酸去铁胺制剂中去铁胺的质量控制。A method for determination of deferoxamine using Fe（ II） by Ultraviolet-visible spectrophotometry was established. In a HAC-Na Ac solution, the deferoxamine could enter into chromogenic reaction with Fe（ II）,and its preparations were determined by Ultraviolet-visible spectrophotometry. The spectrophotometric results were linearly related to the concentration of deferoxamine in the range of 0. 0897 ～ 0. 8074 mg/m L,and the regression equation was Y = 9. 5714X-0. 0069（ R2= 0. 9988）. The recovery and relative standard deviation were 99. 5% and 2. 0%,respectively. The method can be applied to the determination of deferoxamine and its preparations in real pharmaceuticals.

关 键 词：甲磺酸去铁胺 紫外-可见光分光光度法 硫酸亚铁铵 

分 类 号：R917[医药卫生—药物分析学]