头颈部鳞状细胞癌相关基因的生物信息学分析  

Bioinformatics analysis of head and neck squamous cell carcinoma

作　　者：向琳[1] 宫美恒 王苹[1] 尹万忠[1] 杜波[1] 

机构地区：[1]吉林大学第一医院耳鼻咽喉头颈外科,吉林长春130021

出　　处：《中国实验诊断学》2017年第3期381-385,共5页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金子课题(81372900)

摘　　要：目的从基因和分子水平探讨头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)的发生机制。方法从GEO数据库下载人HNSCC相关基因芯片数据,使用BRB-Array Tools软件筛选差异表达基因,采用DAVID、ToppGene、STRING、Cytoscape工具进行生物信息学分析。结果 BRB分析共筛选出263条差异表达基因,其中上调基因134个,下调基因共129个,对其进行生物信息学分析发现COL1A1、MMP9、COL1A2、COL3A1、MMP1等基因调节HNSCC细胞外基质受体相互作用、小细胞肺癌、肿瘤通路等,影响肿瘤的发生发展。结论利用生物信息学分析能有效挖掘基因芯片内在数据,为进一步探讨HNSCC的发生机制提供理论参考。Objective To investigate the mechanism of head and neck squamous cell carcinoma（HNSCC）from the gene and molecular level.Methods Download HNSCC related genes microarray data from GEO database,Screening differentially expressed genes using BRB-Array Tools software.Bioinformatics analysis was carried out using DAVID,ToppGene,STRING and Cytoscape tools.Results Two hundred and sixty-three differentially expressed genes,134 upregulated and 129down-regulated,were screened out by BRB.The COL1A1,MMP9,COL1A2,COL3A1,MMP1 gene,etc regulated ECM-receptor interaction,small cell lung cancer and pathways in cancer.Conclusion The use of bioinformatics to analyze microarray data can provide theoretical reference for in order to further explore the mechanism of HNSCC.

关 键 词：头颈部鳞状细胞癌 差异表达基因 基因芯片 生物信息学 

分 类 号：R739.91[医药卫生—肿瘤]