杠柳毒苷体外抑制胃癌细胞增殖能力的分子共调控网络构建研究  

Construction of Gene-Coregulation-Network for Inhibition Activities of Periplocin on gastric cancer ex vivo

作　　者：王炬玮 臧文远[2] 刁凯[1] 刘海燕[1] 

机构地区：[1]吉林油田总医院血液肿瘤科,吉林松原138000 [2]吉林油田总医院CT科,吉林松原138000

出　　处：《中国实验诊断学》2017年第3期392-395,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨构建杠柳毒苷体外抑制胃癌细胞系SGC-7901细胞增殖能力的分子共调控网络,为解析杠柳毒苷抗肿瘤作用的分子机制提供理论支撑。方法本研究收集美国GEO公开数据库中已收录的所有杠柳毒苷处理胃癌SGC-7901细胞系前后的基因表达谱数据;通过差异表达分析获得经过杠柳毒苷处理后胃癌SGC-7901细胞系表达水平发生显著性改变的基因;通过GO基因功能注释以及KEGG代谢通路富集分析,探究杠柳毒苷作用后表达水平发生显著性改变基因的功能;最后结合TRED数据库中收录的人类转录因子信息,构建分子共调控网络。结果与胃癌SGC-7901细胞系相比,经杠柳毒苷处理后的SGC-7901,其癌细胞中共有1105个基因发生差异表达,其中552个基因上调表达,553个基因下调表达。基因功能分析显示,表达上调基因主要参与葡萄糖代谢、钾离子转运、钠离子转运等生物功能;表达下调基因主要参与同嗜性细胞粘着、氨基酸转运、小GTP酶介导的信号转导等生物功能。KEGG代谢通路富集分析结果显示,差异表达基因主要参与肿瘤相关的诸多代谢通路。最后,基于差异表达基因及TRED数据库中所收录的人类转录因子信息,对差异表达基因进行分子偶联,构建了分子共调控网络。结论基于分子共表达网络,对杠柳毒苷的作用分子机制进行了系统性挖掘。Objective In this study,we constructed a Gene-Coregulation-Network to demonstrate the molecular mechanism of how periplocin inhibits the proliferation of gastric cell line SGC-7901.This study may lay a foundation for underlining the molecular mechanism of the anti-tumor activity of periplocin.Methods By collecting SGC-7901 gene expression profiling data from GEO database,we analyzed the differentially expressed genes.Utilizing GO and KEGG database,we annotated all of the differentially expressed genes.Finally,using transcription factor data provided by TRED database and pearsoncorrelation analysis,we constructed a gene coregulation network.Results Compared with normal SGC-7901 cell lines,1105 genes were differentially expressed in the SGC-7901 cells treated with periplocin,among which 552 genes were up regulated and 553 genes were down regulated.GO annotation results showed that up regulated genes mainly took part in biological functions including glucose metabolism,potassium ion transport and sodium ion transport,and down regulated genes mainly took park in biological functions including hemophilic cell adhesion,amino acid transport,small GTPase mediated signal transduction.KEGG pathway enrichment analysis results showed that,the differentially expressed genes mainly join many cancer-related metabolism pathways.Finally,based on the transcription factor data and pearsoncorrelation analysis,we constructed a gene co-regulation network.Conclusion Based on molecular co-regulation analysis,we systemically mining the molecular mechanism of periplocin.

关 键 词：杠柳毒苷 胃癌SGC-7901细胞系 分子共调控网络 细胞增殖 

分 类 号：R735.2[医药卫生—肿瘤]