PM_(2.5)对小鼠泪膜功能和角膜上皮组织结构的影响  

Effects of PM_(2.5) on tear film function and corneal epithelial structure in mice

作　　者：李娟[1] 丁小艳 王亚虹 叶鳞泓 叶蕾[4] 姜楠[4] 邵毅[4] LI Juan DING Xiao-Yan WANG Ya-Hong YE Lin-Hong YE Lei JIANG Nan SHAO Yi

机构地区：[1]西安市第四医院眼科,陕西省西安市710004 [2]西安市第二医院眼科,陕西省西安市710003 [3]西安市环境监测站,陕西省西安市710054 [4]南昌大学第一附属医院眼科,江西省南昌市330006

出　　处：《眼科新进展》2017年第3期201-204,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81160118;81400372;81400424;81660158);陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04);2017年陕西省创新人才推进计划项目;江西省远航工程(编号:2014022);江西省自然科学基金重大项目(编号:2016ACB21017);江西省青年科学基金(编号:20151BAB215016);江西省科技支撑计划项目(编号:20151BBG70223)~~

摘　　要：目的观察PM_(2.5)对小鼠泪膜功能和角膜上皮组织结构的影响。方法24只雄性6~8周龄BALB/c小鼠,随机分为A、B两组,每组12只。B组采用5 mg·mL^(-1)PM_(2.5)混悬液滴眼,A组采用PBS滴眼,每天4次。分别在干预后1d、4d、7d对各组小鼠进行泪膜功能检测,包括泪液分泌功能、泪膜破裂时间(break-up time,BUT)、荧光素染色(fluorescein staining,FL),并于干预后7d行苏木精-伊红染色观察角膜上皮情况。结果干预后4d、7d,A组泪液分泌量、BUT较干预前无明显变化,差异均无统计学意义(均为P>0.05),而B组泪液分泌量、BUT较干预前明显分别减少和恶化,差异均有统计学意义(均为P<0.05)。干预后7d,A组FL评分较干预前无明显变化,差异无统计学意义(P>0.05),而B组FL评分较干预前明显增加,差异有统计学意义(P=0.003)。干预后7d,A组上皮细胞层数为(4±1)层,而B组上皮细胞层数为(7±1)层,差异有统计学意义(P<0.05)。与A组比较,B组整个角膜FL着染明显增加,角膜表面上皮细胞层损伤,层数增厚。结论 PM_(2.5)会影响小鼠泪膜功能,损伤小鼠角膜上皮的组织结构。Objective To investigate the effects of PM2.5 on tear film function and corneal epithelial structure in mice.Methods Totally 24 male BALB/c mice （24 eyes） were divided into two groups：group A （with PBS eye drops,n=12）,group B （5 mg·mL-1 PM2.5 eye drop group,n=12）.PBS and PM2.5 eye drop were given with four times per day for 7 consecutive days in right eye.Tear secretion level was measured with phenol red thread.Break-up time （BUT） of tear film was tested,and corneal fluorescein staining （FL） was scored before therapy and 1 day,4 days and 7 days after droppings and HE staining was performed 7 days after droppings,respectively.Results There was no significant difference in the tear secretion levels,BUT,FL between the groups A and B before treatment （all P〉0.05）.At 4 days,7 days after treatment with PM2.5,the mean differences of the group B showed all items significantly changed compared with those before treatment （all P〈0.05）.For the group A,there was no statistical change in tear secretion levels,BUT,FL at 7 days after treatment （all P〉0.05）.There were statistical differences in all items between group A and B at each time point （all P〈0.05）.At 7 days after therapy,the mean layers of corneal epithelial cells in the group A （4±1） was significantly lower than that in the group B （7±1）（P〈0.05）.The group B showed that the whole corneal fluorescein staining obviously increased,and corneal epithelial cell layer was thickened.Conclusion PM2.5 can influence tears film function and damage the corneal epithelial structure in mice.

关 键 词：PM2.5 泪膜功能 角膜上皮 小鼠 

分 类 号：R772[医药卫生—眼科] R777[医药卫生—临床医学]