雌二醇对视网膜缺血-再灌注损伤后基质细胞衍生因子-1表达的影响  

Effects of 17β-estradiol on stromal cell-derived factor-1 expression after retinal ischemia-reperfusion injury

作　　者：王业青[1] 张晓梅[1] 董丽[1] 石焕琦[1] 毕文娇 侯文文[1] WANG Ye-Qing ZHANG Xiao-Mei DONG Li SHI Huan-Qi BI Wen- Jiao HOU Wen-Wen

机构地区：[1]哈尔滨医科大学附属第一医院眼科,黑龙江省哈尔滨市150001

出　　处：《眼科新进展》2017年第3期215-219,共5页Recent Advances in Ophthalmology

基　　金：黑龙江省卫生计生委科研课题(编号:2014-299);黑龙江省博士后科研启动金(编号:LBHQ15101)~~

摘　　要：目的通过观察视网膜缺血-再灌注损伤后基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)的表达情况,以及雌二醇对SDF-1的表达及调控作用,研究雌二醇对大鼠视网膜缺血-再灌注损伤的保护作用机制。方法通过升高大鼠眼压的方法建立视网膜缺血-再灌注损伤模型,应用RT-PCR和Western blot方法检测视网膜缺血-再灌注损伤后各时间点(6 h、12 h、24 h)视网膜SDF-1表达情况;腹腔注射雌二醇后观察雌二醇对视网膜缺血-再灌注损伤后SDF-1表达的影响;并且应用雌激素受体拮抗剂ICI 182-780研究雌激素受体对雌二醇诱导视网膜缺血-再灌注损伤后的SDF-1的影响。结果SDF-1 mRNA和蛋白在缺血-再灌注6 h组、缺血-再灌注12 h组和缺血-再灌注24 h组表达均增加,与正常对照组比较差异均有统计学意义(均为P<0.05),并且在缺血-再灌注12 h组SDF-1表达达高峰。雌二醇预处理对视网膜缺血-再灌注具有一定的保护作用;缺血-再灌注+雌二醇组SDF-1 mRNA和蛋白表达增加,与缺血-再灌注对照组及缺血-再灌注+溶剂对照组比较差异均有统计学意义(均为P<0.05)。与缺血-再灌注+雌二醇组相比较,缺血-再灌注+雌二醇+ICI 182-780组SDF-1 mRNA和蛋白表达明显降低,差异均有统计学意义(均为P<0.0 5)。结论雌二醇对视网膜缺血-再灌注损伤的保护作用是通过雌激素受体介导的SDF-1 mRNA和蛋白表达增强实现的。Objective To examine the expression of stromal cell-derived factor-1 （SDF-1） in a rat model of retinal ischemia-reperfusion injury （RIRI）,and investigate the protective effect of 17β-Estradiol （E2） on RIRI and explore the mechanism.Methods The RIRI model was established in Sprague-Dawley rats by increasing the intraocular pressure.Relative expression levels of SDF-1 mRNA and protein in the retina at 6 hours,12 hours and 24 hours following reperfusion was determined by RT-PCR and Western blot,respectively.E2 was administered to investigate the effects of estrogen on SDF-1 expression,and the estrogen receptor antagonist ICI 182-780 was administered to investigate the effect of estrogen receptor on the expression of SDF-1.Results SDF-1 expression in RIRI 6 hours group,12 hours group and 24 hours group was increased compared with normal control group （all P〈0.05）,with maximum expression at RIRI 12 hours group.As expected,pretreatment of RIRI rats with E2 had a protection on RIRI retina;SDF-1 expression was increased in RIRI＋E2 group compared with IR control group and RIRI＋vehicle group （all P〈0.05）.RIRI＋E2＋ICI 182-780 group could decrease SDF-1 expression compared with RIRI＋E2 group（all P〈0.05）.Conclusion E2 offers protection against RIRI by inducing an up-regulation in SDF-1 expression through activation of the estrogen receptor.

关 键 词：视网膜 缺血-再灌注损伤 雌二醇 基质细胞衍生因子-1 

分 类 号：R774.1[医药卫生—眼科]