8种中药提取物在膏霜类化妆品中的抑菌效能研究  

Bacterium inhibition efficacy of eight kinds of herbal extract in cream type cosmetics

作　　者：区梓聪 何秋星[1] 陈佩[1] 方电力 

机构地区：[1]广东药科大学医药化工学院,广东中山528458 [2]广东盛美化妆品有限公司,广东佛山528300

出　　处：《日用化学工业》2017年第3期148-152,158,共6页China Surfactant Detergent & Cosmetics

基　　金：2015年广东药科大学"创新强校工程人才培养模式创新实验区"资助项目(gyzlgc201503);2015年广东药科大学校级教育教学成果奖培育项目;广东省化妆品工程技术研究中心资助项目

摘　　要：采用纤维素酶法提取8种中药(丁香、鱼腥草、虎杖、黄连、大黄、细辛、甘草和黄芩),并采用滤纸片法和试管二倍稀释法研究8种中药提取物的抑菌效能。根据其抑菌作用进行复配研究,参考美国个人护理品委员会(PCPC)的抑菌效能测试方法对膏霜配方进行微生物挑战试验。结果表明,在8种中药提取物中,黄连提取物对金黄色葡萄球菌、铜绿假单胞菌和大肠埃希氏菌的抑制效果最好,其抑菌圈直径分别为(12.4±0.4),(11.4±0.6)和(10.7±0.2)mm,最低抑菌浓度(MIC)分别为31.25,31.25和15.625 g/L。当黄连和黄芩提取物在膏霜配方中的质量分数分别为0.8%和0.6%时,可较强地抑制细菌和真菌的生长,可通过微生物挑战试验。Extracts of eight kinds of herb（ clove,houttuynia,Polygonum cuspidatum,Coptis chinensis,rhubarb,asarum,licorice and scutellaria） were prepared by cellulase method; and their bacterium inhibition efficacy was studied by disc diffusion method and test tube broth dilution method. Formulation study based upon the bacterium inhibition of the aforesaid preservatives was conducted. With reference of the bacterium inhibition efficacy test method of American Personal Care Product Committee（ PCPC）,microbial challenge tests for cream formulation was processed. The test results demonstrated that in the eight kinds of herbal extract,the extract of Coptis chinensis shows best efficacy on Staphylococcus aureus,Pseudomonas aeruginosa and Escherichia coli. Its diameter of inhibition zone is（ 12. 4 ± 0. 4）,（ 11. 4 ± 0. 6） and（ 10. 7 ± 0. 2） mm respectively,and the minimum inhibitory concentration（ MIC） is 31. 25,31. 25 and 15. 625 g/L respectively. When the mass fraction of the extract of Coptis chinensis and scutellaria in cream formula is 0. 8% and 0. 6% respectively,it can inhibit the growth of bacteria and fungi effectively and pass the microbial challenge test meanwhile.

关 键 词：化妆品 中药提取物 抑菌效能 纤维素酶 

分 类 号：TQ658[化学工程—精细化工]