转录因子GATA4对颅面骨发育与矿化的影响  

The role of transcription factor GATA4 incraniofacial bone formation and mineralization

作　　者：周婷婷[1,2] 郭舒瑜 张宇新[1,2] 翁亚娟[1,2] 张梦男[2] 马俊青[1,2] 

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [2]南京医科大学附属口腔医院正畸科,江苏南京210029

出　　处：《口腔生物医学》2017年第1期16-19,共4页Oral Biomedicine

基　　金：国家自然科学基金资助项目(81371179);江苏省杰出青年科学基金资助项目(BK20150048);江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的:通过条件性基因敲除小鼠模型,探讨转录因子GATA4在颅面骨发育与矿化中的作用。方法通过Cre-loxp转基因小鼠技术,将Wnt1-Cre转基因小鼠与GATA4^(fl/fl)转基因小鼠杂交,得到神经嵴细胞特异性GATA4基因敲除小鼠(Wnt1-Cre;GATA4^(fl/fl))。取2周龄雄性GATA4条件敲除小鼠和野生型小鼠各5只处死后应用Micro-CT进行颅盖骨、腭骨和右侧下颌骨的骨形态及骨密度分析。结果:与野生型小鼠相比,GATA4条件敲除小鼠的颅盖骨长度、宽度、鼻额角、腭部宽度、下颌骨长度及高度均明显减小,而腭中缝宽度增大(P<0.05)。下颌骨骨小梁体积分数、骨小梁数量均下降,骨小梁间隔增加(P<0.05)。结论:GATA4参与调控了颅面部骨的发育,对颅面部骨的形成和矿化具有重要调控作用。Objective： To explore the effect of transcription factor GATA4 on bone for^mation and mineralization. Methods： Wntl- Cre mice were mated to GATA4fl/- mice by cre-loxp gene conditional knockout technique,and conditional knockout GATA4 gene of neu-ral crest cell mice （ Wnt1-Cre; GATA4^ ^ ） were produced. 2-week-old GATA4 conditional knockout （CKO） mice and wild type （WT） mice were sacrificed and then Micro-CT was conducted to compare the size and volume of craniofacial bones such as calvarial bone, palate bone and right mandible bone. Results： Compared with WT mice,the skull bone,palate bone and mandible bone of CKO mice were smaller,and the width of midpalatal suture was increased. The mandible of CKO mice also showed decreased BV/TV and Tb. N （P〈0.05） values,and increased Tb.Sp value; Tb.N was not significantly different（P〉0. 0 5 ） . Conclusions： These results indicate an important role of GATA4 in craniofacial bone development and mineralization.

关 键 词：条件性基因敲除 GATA4 颅面部发育 

分 类 号：R783.5[医药卫生—口腔医学]