施氏假单胞菌N2降解1-羟基-2萘甲酸的特性及机理研究  

Study on the Characteristics of 1-hydroxy-2-naphthoic acid degradation by P.stutzeri N2

作　　者：王琰[1,2] 聂麦茜[1] 王菲[1] 聂红云[1] 赵碧洁[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055 [2]陕西省微生物研究所,陕西西安710043

出　　处：《西安建筑科技大学学报（自然科学版）》2017年第1期135-140,共6页Journal of Xi'an University of Architecture & Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(51278405);陕西省科学院应用基础研究基金资助项目(2013k-09)

摘　　要：1-羟基-2-萘甲酸被认定为微生物修复多环芳烃(PAHs)污染过程中最易积累的一种含氧多环芳烃(OPAHs)中间产物,其对大多微生物有较强毒性,并且该物质的积累会抑制微生物对PAHs污染环境的成功修复.本文以施氏假单胞菌N2为对象,研究该菌株对1-羟基-2-萘甲酸的降解代谢能力和特性,并利用GC-MS对降解过程中积累的OPAHs进行鉴定和分析.研究结果表明,N2菌能够以50 mg/L卜羟基-2-萘甲酸为唯一碳源和能源生长,并在以辛烷为共代谢碳源时其生长量和卜羟基-2-萘甲酸的降解率均可提高20%.通过对9种主要中间代谢产物的GC·MS分析,推测脱羧是N2菌降解1-羟基-2-萘甲酸的主要途径.该研究结果将为解决PAHs污染环境修复过程中OPAHs类高毒中间产物积累问题奠定基础.1-hydroxy-2-naphthoic acid was identified as one kind of OPAHs th a t was most easily accumulated during the process of bioremediation. It was toxic to almost all microorganisms, and inhibited successfully restoring PAHs polluted environment. In this study, P. stutzeri N2 , which was selected for its special ability in our previous research, was taken as the object. The capability and characterization of l-hydroxy-2-naphthoic acid degradation by P. stutzeri N2 was studied. At the same t ime , the intermediate metabolisms of l-hydroxy-2-naphthoic acid degraded by P. stutzeri N2 were identified by GC-MS method. The results showed th a t P. stutzeri N2 could utilize 50 mg/L l-hydroxy-2-naphthoic acid as the sole carbon and energy resource. When octane was used as the co-carbon source, the growth increase and degradation rate of P. stutzeri N2 could be improved by 20%. Based on the analysis of nine degradation metabolites by P. stutzeri N2 , decarboxylation was proposed as the main degradation pathway. This study can help lay a foundation for the problem of high-toxic OPAHs accumulation during the process of bioremediation of PAHs polluted environment.

关 键 词：含氧多环芳烃 施氏假单胞菌 1-羟基-2萘甲酸 转化产物 降解途径 

分 类 号：X132[环境科学与工程—环境科学]