HPLC-ELSD法同时测定东北岩高兰果肉中17种氨基酸的含量  

Simultaneous Determination of 17 Amino Acids in Sarcocarp of Northeast Empetrum nigrum by HPLCELSD

作　　者：金司阳 刘寒[1] 郭慧[1] 王振月[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《中国药房》2017年第9期1272-1274,共3页China Pharmacy

摘　　要：目的:建立同时测定东北岩高兰果中17种氨基酸含量的方法。方法:采用高效液相色谱-蒸发光散射检测法。色谱柱为Waters Symmetry C18,流动相为5.0 mmol/L七氟丁酸溶液(含0.7%三氟乙酸)-乙腈(梯度洗脱),流速为2.0 L/min,柱温为37℃,漂移管温度为60℃。结果:甘氨酸、丝氨酸、天冬氨酸、丙氨酸、苏氨酸、谷氨酸、胱氨酸、赖氨酸、组氨酸、精氨酸、脯氨酸、缬氨酸、甲硫氨酸、酪氨酸、异亮氨酸、亮氨酸、苯丙氨酸检测质量浓度线性范围分别为0.005 9~0.187 7、0.008 2~0.262 8、0.010 4~0.332 8、0.007 0~0.222 7、0.009 3~0.297 8、0.011 5~0.367 8、0.009 4~0.300 4、0.011 4~0.365 5、0.012 1~0.388 0、0.013 6~0.435 5、0.009 0~0.287 8、0.009 2~0.293 0、0.011 7~0.373 0、0.014 2~0.453 0、0.010 3~0.328 0、0.010 3~0.328 0、0.012 9~0.413 0mg/m L(r均>0.999 0);精密度、稳定性、重复性试验的RSD≤2.0%;加样回收率为98.0%~101.2%(RSD均<2.0%,n=6)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于东北岩高兰果中17种氨基酸含量的同时测定。OBJECTIVE：To determine the content of 17 amino acid in sarcocarp of northeast Empetrum nigrum simultaneously. METHODS：HPLC-ELSD method was adopted. The determination was performed on Waters Symmetry C18 column with mobile phase consisted of 5.0 mmol/L heptafluorobutyric acid solution（containing 0.7% trifluoroacetic acid）-acetonitrile（gradient elution）at the flow rate of 2.0 L/min,the column temperature was 37 ℃,the drift tube temperature was set at 60 ℃. RESULTS：The linear ranges of glycine,serine,aminosuccinic acid,aminopropionic acid,threonine,glutamic acid,cystinol,diaminocaproic acid,histidine,arginine,proline,valine,methionine,oxyphenylaminopropionic acid,isoleucine,aminocaproic acid,phenylalanine were0.005 9-0.187 7,0.008 2-0.262 8,0.010 4-0.332 8,0.007 0-0.222 7,0.009 3-0.297 8,0.011 5-0.367 8,0.009 4-0.300 4,0.011 4-0.365 5,0.012 1-0.388 0,0.013 6-0.435 5,0.009 0-0.287 8,0.009 2-0.293 0,0.011 7-0.373 0,0.014 2-0.453 0,0.010 3-0.328 0,0.010 3-0.328 0 and 0.012 9-0.413 0 mg/m L（all r〉0.999 0）. RSDs of precision,stability and repeatability tests were not all less than 2.0%. Recoveries were 98.0%-101.2%（RSD〈2.0%,n=6）. CONCLUSIONS：This method is simple,precision,stable and repeatable,and can be used for simultaneous determination of 17 amino acid in sarcocarp of northeast E. nigrum.

关 键 词：东北岩高兰 高效液相色谱法 蒸发光散射检测 氨基酸 

分 类 号：R917[医药卫生—药物分析学]