原花青素对淀粉样蛋白Aβ_(25-35)诱导的SH-SY5Y细胞毒性的影响  

The influences of proanthocyanidin on amyloid beta fragment Aβ_(25-35) induced cytotoxicity in SH-SY5Y cells

作　　者：周亚盼 张小强[1,2] 曲志华[1,2] 张妍[1,2] 梁晓瑜[1,2] 

机构地区：[1]东南大学公共卫生学院,江苏南京210009 [2]东南大学环境医学工程教育部重点实验室,江苏南京210009

出　　处：《癌变．畸变．突变》2017年第2期91-95,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：2016年江苏省普通高校研究生科研创新计划资助项目资助(SJZZ16_0034);江苏省卫生厅预防医学科研基金(Y2013070)

摘　　要：目的:探讨原花青素对淀粉样蛋白片段Aβ_(25-35)染毒的SH-SY5Y细胞存活、氧化应激及其分泌淀粉样蛋白Aβ_(1-42)和可溶性淀粉样蛋白sAPPα水平的影响。方法:分别以不同浓度(0.1、0.5、1.0、5.0μg/m L)原花青素和不同浓度(0.1、1.0、10.0、20.0μmol/L)的Aβ_(25-35)作用SH-SY5Y细胞24 h后用MTT法检测细胞活力,进一步以1.0μmol/L Aβ_(25-35)染毒细胞,建立细胞损伤模型,不同浓度(0.1、0.5、1.0、5.0μg/m L)原花青素干预24 h,用MTT法检测细胞活力,ELISA法检测细胞分泌Aβ_(1-42)水平及s APPα水平,TBA法测定细胞内丙二醛(MDA)含量,WST-8法测定细胞内总超氧化物歧化酶(SOD)活力。结果:与对照组相比,不同浓度原花青素作用24 h后,细胞存活率差异均无统计学意义(P均>0.05),而不同浓度Aβ_(25-35)均使SH-SY5Y细胞活力降低(P<0.01),其中1.0μmol/L Aβ_(25-35)对细胞活力的抑制作用最强(P<0.01)。以1.0μmol/L Aβ_(25-35)染毒细胞,Aβ_(1-42)分泌水平升高(P<0.01),s APPα水平降低(P<0.01),细胞内MDA含量升高(P<0.01),总SOD活力降低(P<0.01)。与1.0μmol/L Aβ_(25-35)染毒组比较,0.1、0.5、1.0、5.0μg/m L原花青素干预可提高各组细胞活力(P<0.05),降低Aβ_(1-42)分泌水平(P<0.01);0.5、1.0、5.0μg/m L原花青素干预可提高细胞分泌s APPα水平(P<0.05),降低细胞内MDA含量(P<0.05),增强总SOD活力(P<0.05)。结论:原花青素可减轻Aβ_(25-35)诱导的SH-SY5Y细胞Aβ负荷,并减轻细胞氧化损伤。OBJECTIVE：and soluble amyloid precursor protein sAPPα which were induced by amyloid protein fragment Aβ25-35 in SH-SY5Y cells was investigated.METHODS：SH-SY5Y cells were treated with Aβ25-35 of different concentrations （0.1,1.0,10.0 and 20.0μmol/L） for 24 hours. Cell viability was measured by MTT assay. Cell injury models were built with 1.0μmol/L Aβ25-35,with proanthocyanidin of different concentrations added to cell cultures and incubated for 24h. Concentrations of Aβ1-42 and sAPPαin cell supernatant were determined by using ELISA. Intracellular content MDA and total SOD were determined by the method of TBA test and WST-8,respectively.RESULTS：Compared with the control group,cells treated with Aβ25-35 （1μmol/L） hadsignificantly declined cell viability （P〈0.01）,increased Aβ1-42 level （P〈0.01）,declined sAPPα concentration （P〈0.01）, increased intracellular MDA content （P〈0.01）,and decreased total SOD vitality （P〈0.01）. Compared with the Aβ25-35 injury cell models,proanthocyanidin （0.1,0.5,1.0 and 5.0μg/mL） significantly improved cell viability （P〈0.05）,decreased the secretion of Aβ1-42 （P〈0.01）. Proanthocyanidin （0.5,1.0 and 5.0μg/mL） increased sAPPα concentration （P〈0.05）,decreased MDA content （P〈0.05）,and strengthened total SOD vitality （P〈0.05）. CONCLUSION：Proanthocyanidin alleviated the Aβ burden a nd o xidative s tress i n S H-SY5Y c ells w hich w ere i nduced b y Aβ25-35. The influence of proanthocyanidins on oxidative stress,secretion of amyloid protein Aβ1-42.

关 键 词：原花青素 AΒ1-42 sAPPα 细胞毒性 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]