高血压小鼠心肌肥厚过程中心肌组织Prx-2、8-OHdG蛋白表达变化及意义  

Expression changes of Prx-2 and 8-OHd G protein in mice with hypertension-induced myocardial hypertrophy

作　　者：王若楠 范玉磊 梁婷婷 耿小番 刘璇 姚程 孙影 WANG Ruonan FAN Yulei LIANG Tingting GENG Xiaofan LIU Xuan YAO Cheng SUN Ying(School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063000, Chin)

机构地区：[1]华北理工大学基础医学院,河北唐山063000

出　　处：《山东医药》2017年第4期5-8,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81200188);国家级大学生创新创业训练计划项目(201410081023)

摘　　要：目的探讨高血压小鼠心肌肥厚过程中心肌组织硫氧环蛋白过氧化物酶2(Prx-2)、8-羟基脱氧鸟嘌呤(8-OHd G)蛋白表达变化及其意义。方法将60只雄性C57BL/6N小鼠分为观察组40只和对照组20只,观察组采用两肾一夹(2K1C)法制备高血压心肌肥厚模型,对照组只分离左肾动脉,不放置肾动脉夹。两组分别于术前和术后2、4、8、12周测量尾动脉收缩压;术后2、4、8、12周分别随机选择10只观察组小鼠及5只对照组小鼠处死,计算全心质量指数和左心室质量指数,分别采用免疫印迹法、免疫组化法检测左心室心肌组织Prx-2、8-OHd G蛋白表达;分析观察组术后各时间点Prx-2、8-OHd G蛋白表达的关系。结果两组术前尾动脉收缩压比较无明显统计学差异(P>0.05),观察组术后2、4、8、12周尾动脉收缩压、全心质量指数和左心室质量指数均呈升高趋势,且均高于对照组同时间点(P均<0.05);证实建模成功。观察组术后2、4、8、12周左心室心肌组织Prx-2蛋白相对表达量及8-OHd G蛋白表达均呈升高趋势,且均高于对照组同时间点(P均<0.05)。观察组术后2、4、8、12周左心室心肌组织8-OHd G和Prx-2蛋白表达均呈正相关(r分别为0.95、0.97、0.97和0.95,P均<0.01)。结论高血压小鼠心肌肥厚过程中左心室心肌组织Prx-2、8-OHd G蛋白表达均升高,二者分别通过抗氧化和促氧化作用参与心肌肥厚的发生与发展。Objective To investigate the expression and significance of peroxiredoxin-2 （ Prx-2） and 8-hydroxy-2′-de-oxyguanosine （8-OHdG） in mice with hypertension-induced myocardial hypertrophy .Methods Sixty male mice were ran-domly divided into the observation group （n=40） and control group （n=20）.Mice in the observation group received 2-kidney1-clip （2K1C） to make the models of mice with hypertension-induced myocardial hypertrophy , while there was no silver clip in mice of the control group .The systolic blood pressure （ SBP） was measured in all mice before the operation , and 2, 4, 8 and 12 weeks after 2K1C.Ten mice in observation group and five mice in the control group were scarified at 2, 4, 8 and 12 weeks after surgery to measure the whole heart quality index and left ventricle quality index .Prx-2 and 8-OHdG protein expression was detected by Western blotting and Immunohistochemistry .And then, we analyzed the correla-tion between Prx-2 and 8-OHdG.Results No significant difference was found in the preoperative SBP between the control and observation groups （P〉0.05）.Compared with the control group , SBP, whole heart quality index and left ventricle quality index was increased in the observation group at 2, 4, 8 and 12 weeks after surgery, indicating that the hypertension model was successful （all P〈0.05）.The expression levels of 8-OHdG and Prx-2 protein in the observation group at 2, 4, 8 and 12 weeks after surgery were significantly higher than those of the control group （all P〈0.05）, and the expression levels of 8-OHdG and Prx-2 protein was positively correlated with each other （ r=0.95, 0.97, 0.97 and 0.95, P〈0.05）.Conclusion The expression levels of Prx-2 and 8-OHdG in mice with hypertension-induced myocardial hypertro-phy are increased , and they participate the occurrence and development of myocardial hypertrophy through oxidation and anti-oxidation.

关 键 词：高血压 硫氧环蛋白过氧化物酶2 8-羟基脱氧鸟嘌呤 心肌肥厚 活性氧 小鼠 

分 类 号：R151.41[医药卫生—营养与食品卫生学]