高效液相色谱-质谱法测定啤酒中伏马毒素含量  

Determination of Fumonisin in beer by High Performance Lquid Chromatography-Mass Spectrometry

作　　者：汪琼[1] 严付华[1] 文刚[1] WANG Qiong YAN Fu-hua WEN Gang(Changsha Center of Disease Control and Prevention, 410004, Changsha Hunan)

机构地区：[1]湖南省长沙市疾病预防控制中心,410004

出　　处：《首都食品与医药》2017年第6期10-11,共2页Capital Food Medicine

摘　　要：目的探讨液相色谱-质谱法测定啤酒中3种伏马毒素的可行性。方法样品经乙腈提取,过Multi Sep~?211 Fum净化柱净化后,采用UPLC-MS/MS电喷雾正离子ESI+,以多离子反应监测(MRM)模式检测,外标法定量。结果 3种伏马毒素线性范围为2.001μg/L^100.12μg/L,线性相关系数在0.9990~0.9996之间,检出限在0.1~0.3μg/kg,3个不同水平的平均加标回收率在82.6%~91.5%间,相对标准偏差(RSD)均<6.08%。结论该方法有干扰少,操作简便,快速准确等优点,为检测啤酒中此类毒素提供了一种新的方法。Object ive To investigate the feasible for the determination of three fumonisin in beer samples by using high performance liquid chromatography-mass spectrometric. Methods The three fumonisin in beer samples were extracted with zcetonitrile.After purified by MuhiSep 211 Fum column, these target analytes were detected by UPLC-MS/MS- ESI＋（MRM model） and quantified with external standard method.Results The calibration curves of three fumonisin were in the range of 2.001 p~ g/L - 100.12 Ix g/L, with the correlation coefficients（r2） in the range of 0.9990 N 0.9996, the limit of defection of this method was in the range of 0.1 - 0.3 I~ g/kg, the recoveries of three different spiked concentration were in the range of 82.6% - 91.5%, and the RSDs were less than 6.08%.Conculsion The new method possess many advantages, such as less interference from impurities, easy operation, fast and accurate, which is a good potential method for the detection of fumonisin.

关 键 词：啤酒 伏马毒素 超高效液相色谱-质谱 

分 类 号：O657.63[理学—分析化学] TS262.5[理学—化学]