IL-8受体介导c-Jun和Ets-1异常调控MMP-9促进胃癌细胞侵袭  

IL-8 receptor mediated c-Jun and Ets-1 abnormal promoted gastric cancer cells metastasis through MMP-9

作　　者：陈惠新[1] 欧志英 陈丽莎[1] 徐永成[1] 曾书君[1] 罗程[1] 余志金[1] 

机构地区：[1]惠州市中心人民医院消化内科,广东惠州516001 [2]广州市妇女儿童医疗中心,广东广州510623

出　　处：《南京医科大学学报（自然科学版）》2017年第2期184-188,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：广东省自然科学基金面上项目(S201310011923);惠州市科技计划项目(2013Y009)

摘　　要：目的:探讨白介素-8(IL-8)受体促进胃癌侵袭所介导的分子机制。方法:胃癌细胞培养后分别转染空白质粒pc DNA3.1(+)、针对IL-8受体的反义表达质粒pc DNA3.1(+)/as-IL-8G和针对IL-8受体的正义表达质粒pc DNA3.1(+)/s-IL-8G,Western blot检测蛋白表达,细胞侵袭力用相对侵袭细胞数量表示。结果:低表达IL-8受体的胃癌细胞侵袭能力显著减弱,且细胞内c-Jun、Ets-1、MMP-9的蛋白水平显著下降;而高表达IL-8受体的胃癌细胞侵袭能力则显著增强,且细胞内c-Jun、Ets-1、MMP-9的蛋白水平显著上升。用硫代磷酸化修饰的反义寡聚脱氧核苷酸阻断该细胞内c-Jun或Ets-1的表达后,MMP-9的蛋白表达水平显著降低,并伴随细胞侵袭能力明显下降。结论:IL-8受体通过介导c-Jun和Ets-1异常调控MMP-9促进了胃癌细胞的侵袭过程。Objective：To explore the molecular mechanism in which IL-8 receptor promoted gastric cancer metastasis. Methods：Gastric cancer cells were cultured and transfected with IL-8 receptor antisense expression plasmid pc DNA3.1（＋）/as-IL-8G and IL-8receptor sense expression plasmid pc DNA3.1（＋）/s-IL-8G. Protein expression was detected by Western blotting. Cell invasion was performed in Transwell and the invasion capacity was expressed by the relative invasive cell number. Results：Gastric cancer cells with lower expression of IL-8 receptor had significantly reduced invasion ability,and the expression of c-Jun,Ets-1 and MMP-9decreased correspondingly,while higher expression of IL-8 receptor markedly enhanced the invasion capacity of gastric cancer cells,and the expression of c-Jun,Ets-1 and MMP-9 increased significantly. MMP-9 protein expression level decreased significantly after blocking the c-Jun or Ets-1 expression by phosphorylated modification antisense oligonucleotide technology,and the cell invasion ability decreased obviously at the same time. Conclusion：IL-8 receptor promoted gastric cancer cells metastasis through abnormal cJun and Ets-1-mediated MMP-9.

关 键 词：胃癌 侵袭 IL-8受体 C-JUN ETS-1 MMP-9 

分 类 号：R735.2[医药卫生—肿瘤]