大鼠脑缺血再灌注早期血脑屏障通透性的变化  

Changes of blood-brain barrier permeability in early stage of cerebral ischemia-reperfusion injury in rats

作　　者：杨明坤[1,2] 司明文[3] 赵宝东[1] 

机构地区：[1]辽宁医学院解剖学教研室,锦州121001 [2]茌平县人民医院神经内科,聊城252100 [3]聊城市人民医院急诊科,聊城252001

出　　处：《中华老年多器官疾病杂志》2017年第3期212-216,共5页Chinese Journal of Multiple Organ Diseases in the Elderly

摘　　要：目的探讨大鼠脑缺血再灌注早期血脑屏障(BBB)通透性的变化。方法采用随机数字表法,将大鼠分为6组:假手术(Sham)组、缺血1 h组,缺血2 h组、缺血2 h再灌注0.5 h组、缺血2 h再灌注1 h组和缺血2 h再灌注2 h组。采用大脑中动脉线栓法制作局灶性脑缺血再灌注模型,透射电子显微镜下观察,并经鼠尾静脉注射I^(125)-神经生长因子(I^(125)-NGF),观察脑组织中I^(125)-NGF渗入量的变化。采用脑组织冠状冰冻切片放射自显影技术观察I^(125)-NGF在脑组织中的范围。结果与Sham组比较,缺血1 h组,缺血2 h组、缺血2 h再灌注0.5 h组、缺血2 h再灌注1 h组和缺血2 h再灌注2 h组大鼠的^(125)I-NGFγ计数均显著增高,差异具有统计学意义(P<0.05)。与Sham组比较,缺血2 h组内皮细胞管腔面的微绒毛数目明显增多,内皮细胞轻度皱缩,胞质中胞饮小泡正在形成,内皮细胞胞质中部分线粒体轻度肿胀,星彤胶质细胞足突轻度肿胀,细胞外间隙轻度扩大。缺血2 h再灌注2 h组内皮细胞核明显皱缩,内皮细胞线粒体肿胀模糊,基质膜增宽,密度变淡,星形胶质细胞足突肿胀,细胞外间隙扩大,水肿液积聚。Sham组脑室部有少量的I^(125)-NGF渗入;缺血2 h组的脑室和部分皮层损伤中心有I^(125)-NGF渗入;缺血2 h再灌注2 h组的脑室、损伤侧大脑皮层及皮层下组织均有较多I^(125)-NGF渗入。结论大鼠脑缺血再灌注早期有BBB的破坏,随缺血再灌注时间延长,BBB通透性加剧。Objective To study the changes of the permeability of blood-brain barrier （ BBB ） in the early stage after cerebral ischemia-reperfusion （I/R） injury in rats.Methods Thirty rats were randomly divided into 6 groups：sham group, ischemia for 1 h group （I1h）, ischemia for 2 h group （I2h）, ischemia for 2 h followed by reperfusion for 0.5 h （I2h/R0.5h）, ischemia for 2 h followed by reperfusion for 1 h （I2h/R1h）, and ischemia for 2 h and then reperfusion for 2 h （I2h/R2h）.The cerebral ischemia-reperfusion model of middle cerebral artery occlusion （ MCAO） was established by middle cerebral artery occlusion .The BBB structure was observed with transmission electron microscopy .After I^125 labeled nerve growth factor （ I^125-NGF） was injected into the rats by tail vein, its permeation through the BBB was determined by counting γ-rays.Autoradiography was used to observe the distribution of I^125-NGF in the involved areas in the frozen section of brain coronal tissues .Results Compared with that in rats of the sham group , the content of I^125-NGF was significantly higher in the rats from the I1h, I2h, I2h/R0.5h, I2h/R1h, and I2h/R2h groups （P〈0.05）.Compared with sham group , the I2h group displayed that the number of microvilli on the luminal surface of endothelial cells was increased significantly , and the endothelial cells were slightly shrunken , with the pinocytotic vesicles formed in the cytoplasm and slightly swollen mitochondria .And the astrocytes were observed with mild swollen foot processes and wilder extracellular space .In the I2h/R2h group, the endothelial cells were swollen and blurred , with wrinkled nucleus and larger gap , and the astrocytes were with mild swollen foot processes , wilder extracellular space , and accumulated edema fluid .I^125-NGF was slightly infiltrated into the ventricle of the sham group, moderately into the ventricle and involved cortex of the I 2h group, but largely into the ventricle , involved cortex and subcortical tissues in the I2h/R

关 键 词：缺血再灌注 血脑屏障 I^125-神经生长因子 

分 类 号：R743[医药卫生—神经病学与精神病学]