大白菜抗/感干烧心材料CNGCs编码基因的差异解析  

Differential Analysis of Genes Coding for Cyclic-Nucleotide Gated Channels in Two Chinese Cabbage Inbred Lines

作　　者：刘栓桃[1] 张志刚[1] 李巧云[1] 王晓[1] 王立华[1] 赵智中[1] 王淑芬[1] 徐文玲[1] 刘贤娴[1] 刘辰[1] 

机构地区：[1]山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南250100

出　　处：《山东农业科学》2017年第3期10-15,共6页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2014CM021;ZR2015YL073);山东省农业良种工程2014项目"十字花科名产蔬菜新品种选育"(2014LNK-96-PZTP-10);山东省农业科学院青年基金项目(2014QNM11);山东省农业科学院创新工程项目(CXGC2016A11)

摘　　要：在植物中,环腺苷酸门通道(CNGCs)蛋白由一个大的基因家族编码,其参与K+、Na+和Ca2+等阳离子的非选择性吸收和转运,其中钙离子(Ca2+)是植物生长发育必需的营养元素和信号调节因子。在大白菜中,Ca2+缺乏易造成干烧心的发生,不同大白菜品种对干烧心的易感程度存在显著差异。本研究以极端抗/感干烧心大白菜自交系He102与06-247为试材,采用全基因组重测序的方法筛选到了6个编码CNGCs的结构变异基因,分别是Bra003081、Bra022702、Bra032132、Bra024067、Bra021265、Bra031529,其分别与拟南芥CNGCs成员1、4、6、9、19、20同源。对6个大白菜CNGCs的潜在跨膜区进行了预测,发现这些CNGCs包含5~8个跨膜区域,C-端均位于胞质一侧。对编码蛋白氨基酸序列进行了比较,结果表明,来自06-247的Bra003081和Bra031529的C-端分别缺失389个氨基酸残基和多出43个氨基酸残基;Bra032132和Bra024067的变异也发生在06-247中,它们的C-端发生了2~3个氨基酸残基的插入或缺失;Bra021265和Bra022702的变异发生在He102中,前者是可变剪切,后者在N-端第31和第49氨基酸残基处分别缺失和插入一个氨基酸残基。易感干烧心材料06-247中四个突变CNGCs的突变位点都在编码蛋白的C-端,这有可能影响CNGCs的功能。该试验结果为下一步研究CNGCs基因功能、开发特异性标记、鉴定大白菜干烧心与CNGCs突变的关系奠定了基础。In plants,cyclic-nucleotide gated channels（CNGCs） are non-selective cation channels encoded by a large gene family.They can uptake and transport most cations such as Na^＋,K^＋and Ca^2＋,among which,Ca^2＋ plays pivotal roles in the physiology and biochemistry of all plants.Calcium deficiency often causes tip-burn in Chinese cabbage.Different genotypes of Chinese cabbage have obvious differences in the sensitivity to tip-burn.In this study,re-sequencing was conducted in two genotypes of Chinese cabbage inbred lines,He102 and 06-247,which showed significant differences in tip-burn reaction.Six genes coding for CNGCs were screened out and showed coding-region differences between two lines.They wereBra003081,Bra022702,Bra032132,Bra024067,Bra021265 and Bra031529,which showed homologous to Arabidopsis CNGC1,4,6,9,19,20 respectively.The potential transmembrane domains were predicted.These CNGCs contained 5 ~ 8 transmembrane domains and their C-terminals were all located in the inside of cell membrane.Amino acid sequence alignment showed that,Bra003081 and Bra031529,which mutated in06-247,showed C-terminal large fragment deletion and small fragment elongation respectively; Bra032132 and Bra024067,also mutated in 06-247,showed only 2 to 3 amino acid insertion or deletion in C-terminal region; the mutation of Bra021265 and Bra022702 appeared in He102,in which,Bra021265 was alternative-splicing mutation while Bra022702 was single amino acid insertion/deletion mutation in N-terminal region.All the mutations of CNGCs in 06-247 appeared in the C-terminal region,which might influence their functions.The results provided clues for studying gene function of CNGCs,developing site-specific markers and exploring their relations to tip-burn in Chinese cabbage.

关 键 词：大白菜 干烧心 环腺苷酸门通道 基因突变 

分 类 号：S634.101[农业科学—蔬菜学]