外加直流电场对人晶状体上皮细胞移行和增殖活力的影响  

Effect of the electric field on the migration and proliferation of human lens epithelial cells

作　　者：韩静[1] 刘珺[1] 严宏[1] 闫小龙[2] 

机构地区：[1]第四军医大学唐都医院眼科,中国陕西省西安市710038 [2]第四军医大学唐都医院胸外科,中国陕西省西安市710038

出　　处：《国际眼科杂志》2017年第4期619-622,共4页International Eye Science

基　　金：国家自然科学基金青年项目(No.81200617);陕西省自然科学基金(No.2012JM4055)~~

摘　　要：目的:观察外加直流电场对人晶状体上皮细胞(lens epithelial cell,LEC)移行和增殖活力的影响。方法:将培养的人晶状体上皮细胞系HLE-B3细胞暴露于电场强度为100、200、400mV/mm的直流电场中,未受电场暴露的细胞作为正常对照组。倒置显微镜观察并记录电场暴露前及暴露后的细胞图像,并计数细胞数目;流式细胞仪检测电场暴露前及暴露24h后的细胞凋亡率和细胞周期。结果:强度为400mV/mm的电场暴露3h后,HLE-B3细胞呈现朝向电场阴极一侧的定向移行。电场持续暴露后,HLE-B3细胞数逐渐减少,在电场作用6h和12h时分别较正常对照组减少12.6%和18.6%,差异均具有统计学意义(P<0.05)。100、200、400mV/mm电场暴露24h后,HLE-B3细胞凋亡率分别为(9.2±1.9)%、(23.9±2.6)%、(54±2.5)%,与正常对照组相比明显增加(P<0.05);细胞周期检测结果显示,HLE-B3细胞进入G_2/M期的比例随电场强度增加逐渐上升,其中200mV/mm和400mV/mm电场中G_((2))/M期细胞比例分别为(13.8±2.2)%和(15.6±2.5)%,与正常对照组相比差异具有统计学意义(P<0.05)。结论:外加直流电场可诱导HLE-B3细胞的定向移行,随电场作用时间的延长和电场强度增加,细胞生长受到抑制,同时伴随细胞凋亡增加和细胞周期G_2/M期阻滞。·AIM： To observe the effect of applied direct electric field （ EF） on the migration, cell growth, apoptosis and cell cycle of human lens epithelial cells （ hLECs） . ·METHODS： HLE - B3 cells were exposed to EF at 100mV/mm, 200mV/mm and 400mV/mm, respectively. Cells without EF - exposure were treated as normal controls. Photos of HLE-B3 cells were captured before and after EF - exposure, and the cell numbers were calculated. Apoptosis and cell cycle were detected with flow cytometry after EF-exposure for 24h. ·RESULTS： After exposure to EF at 400mV/mm for 3h, HLE-B3 cells showed directed migration to the cathode. The cell number of HLE-B3 cells decreased gradually with continuous EF-exposure, and decreased by 12. 6%at 6h and by 18.6% at 12h, respectively （P〈0.05）. After exposure to EF at 100mV/mm, 200mV/mm and 400mV/mm for 24h, the apoptosis rates of HLE - B3 cells increased dramatically compared to that of the normal control, which were （9. 2±1. 9）%, （23. 9±2. 6）% and （54±2. 5）%, respectively （P〈0. 05）. Accordingly, the results of flow cytometry showed that cell numbers in G2/M phase were increased after EF-exposure, which were （13. 8±2. 2）% and （15. 6±2. 5）% at 200mV/mm and 400mV/mm, respectively （P〈0. 05）. ·CONCLUSION： Applied direct EF induces directed migration of HLE-B3 cells and inhibits the cell growth with the prolongation of the EF-exposure time. EF also induces cell apoptosis and G2/M phase inhibition of the cell cycle in HLE-B3 cells.

关 键 词：电场 人晶状体上皮细胞 细胞移行 凋亡 细胞周期 

分 类 号：R776.1[医药卫生—眼科]