盐酸小檗碱对颅脑外伤模型小鼠自噬和β-分泌酶影响的初步研究  

Effect of berberine chloride on autophagy and β-secretase level in mice with traumatic brain injury

作　　者：黄树宣 朱飞奇[2] 朱瑾华[1] 刘远[1] 陈淳淳[1] 林伟丰[1] 裴中[3] 

机构地区：[1]汕头大学医学院附属粤北人民医院神经内科,韶关512026 [2]深圳大学附属罗湖医院神经内科,深圳518001 [3]中山大学附属第一医院神经内科,广州510080

出　　处：《中华神经医学杂志》2017年第3期251-255,共5页Chinese Journal of Neuromedicine

基　　金：（1）基金项目：广东省自然科学基金（S2013010015786） （2）基金项目：韶关市科技计划项目[（韶科（卫）2010-01）]

摘　　要：目的观察小鼠颅脑外伤后自噬活化和β-分泌酶（BACZ）的变化情况，初步探讨盐酸小檗碱对自噬和BACE的影响。方法健康雌性C57BL／6小鼠18只按随机数字表法分为对照组、模型组、小檗碱组，每组6只，后2组小鼠采用自由落体撞击法建立创伤性脑损伤（TBI）模型，小檗碱组小鼠建模后1d通过灌胃给予盐酸小檗碱（50mg／kg），每天1次，共21d。免疫荧光染色检测3组小鼠损伤侧皮层和丘脑微管相关蛋白1轻链3（LC31以及损伤侧皮层BACE的表达情况。结果建模后21d，3组小鼠损伤侧皮层和丘脑LC3的表达以及损伤侧皮层BACE的表达（对照组、模型组、小檗碱组分别为0．02±0．01、0．06±0．02、0．04±0．01）比较差异均有统计学意义（P〈0．05）。与对照组比较．模型组小鼠损伤侧皮层和丘脑LC3的表达以及模型组、小檗碱组小鼠损伤侧皮层BACE的表达增加，差异有统计学意义（P〈0．05）。与模型组比较，小檗碱组小鼠损伤侧皮层和丘脑LC3的表达以及损伤侧皮层BACE的表达减少，差异有统计学意义（P〈0．05）。结论颅脑外伤可以导致损伤侧皮层和丘脑自噬的活化及损伤侧皮层BACE的过度表达，盐酸小檗碱能够显著抑制这种变化。Objective To investigate the effect of berberine chloride on autophagy and β-secretase （BACE） level in mice with traumatic brain injury （TBI）. Methods Eighteen female healthy C57BL/6 mice were randomly divided into three groups： control group, model group and berberine group （n=6）. TB1 models in the later two groups were established by a weight-drop hitting device and mice in berberine group were administered intragastricly with berberine chloride （50 mg/kg） once daily for 21 d. lmmunofluorescent staining were used to assess LC3 and BACE expressions in ipsilateral cortex or thalamus, and then, their mean fluorescence intensities were calculated and compared among these three groups. Results LC3 expression in the ipsilateral cortex and thalamus and BACE expression in the ipsilateral cortex （0.02±0.01, 0.06±0.02 and 0.04±0.01 in the control group, model group and berberine group） showed significant difference among the three groups （P〈0.05）： LC3 expression in ipsilateral cortex and thalamus and BACE expression in the ipsilateral cortex of the model group were significantly increased as compared with those of the control group （P〈0.05）; the LC3 expression in the ipsilateral cortex and thalamus and BACE expression in the ipsilateral cortex of the model group were significantly decreased as compared with those of the berberine group （P〈0.05）. Conclusion Autophagy is over-activated in the ipsolateral cortex and thalamus and BACE is over-activated in the ipsolateral cortex after TBI, and these changes are significantly suppressed by berberine chloride.

关 键 词：颅脑外伤 盐酸小檗碱 自噬活化 Β-分泌酶 

分 类 号：R-332[医药卫生] R651.15