EGCG和EGCG-3Me对根管牙本质粘接界面稳定性的作用  

The effects of EGCG and EGCG-3 Me on the bonding stability of dentin-adhensive to intraradicular dentin

作　　者：余昊翰 张凌 李芳 刘正雅 李银花[2] 陈吉华 

机构地区：[1]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医院修复科,西安710032 [2]国家植物功能成分利用工程技术研究中心,湖南农业大学茶学教育部重点实验室

出　　处：《实用口腔医学杂志》2017年第2期174-178,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:51373198,81130078,81571019);陕西省科技统筹创新工程计划项目(编号:2015KTCL03-08);长江学者和创新团队发展计划(编号:IRT13051)

摘　　要：目的:评价表没食子儿茶素没食子酸酯(EGCG)及其甲基化修饰物(EGCG-3Me)改性粘接剂对根管牙本质粘接界面稳定性的作用。方法:将质量浓度为400μg/ml的EGCG及EGCG-3Me添加到全酸蚀粘接剂Single Bond 2(SB2)中,制备改性粘接剂E-SB2及E3-SB2,SB2为对照组。激光共聚焦显微镜和分光光度法检测改性粘接剂抗粪肠球菌的性能。微拉曼光谱仪检测粘接剂双键转化率。制备纤维桩粘接试件,用于即刻和老化后的微推出实验。结果:改性粘接剂可以抑制粪肠球菌生物膜形成,且EGCG-3Me作用更显著。改性粘接剂与SB2的双键转化率和即刻微推出粘接强度差异无显著性(P>0.05)。老化后改性粘接剂的微推出粘接强度显著高于SB2(P<0.05)。结论:EGCG和EGCG-3Me改性的粘接剂均可抑制粪肠球菌增殖并提高树脂-根管牙本质粘接界面稳定性,EGCG-3Me抗菌性能较佳。Objective： To evaluate the effect of epigallocatechin- 3- gallate （EGCG） and epigallocatechin- 3- O- （ 3- O- methyl ） - gallate （EGCG-3Me） on the anti-bacterial effect and the stability of intraradicular dentin-adhesive interface. Methods： EGCG and EGCG- 3Me with the concentration of 400 μg/ml were incorporated into Single Bond 2（SB2） respectively to obtain 2 modified adhesives E-SB2 and E3-SB2. Confocal laser scanning microscopy（CLSM） and ultraviolet spectrophotometry were used to evaluate the anti-bacterial effect of the modified adhesives. Micro- Raman spectrum was used to test the degree of conversion （ DC ） of the adhesives. Push- out bond strength test was conducted to examine the immediate bond strength and the bond strength after themocycling. Results： E-SB2 and E3-SB2 both showed inhibiting effect on the proliferation of E. faecalis, while E3-SB2 performed stronger inhibiting effect. DC and the immediate push-out bond strength of SB2 were not decreased with the incorporation of EGCG or EGCG-3Me （P 〉 0.05）. E-SB2 and E3-SB2 showed significantly higher push-out bond strengths than that of SB2 （P 〈 0.05 ） after themocycling. Conclusion： EGCG and EGCG-3Me modified adhesives have anti-bacterial effect and can enhance the stability of bonding between intraradicular dentin and adhesive, EGCG- 3 Me may have stronger anti- bacterial effect.

关 键 词：表没食子儿茶素没食子酸酯(EGCG) 甲基化表没食子儿茶素没食子酸酯(EGCG-3Me) 抗菌 双键转化率 微推出粘接强度 冷热循环 

分 类 号：R783.1[医药卫生—口腔医学]