Wnt1在口腔黏膜下纤维性变中的表达  

The expression of wntl in oral submucous fibrosis

作　　者：邱喜丽 许春姣[2] 王璐[3] 吕逢源[3] 刘婷婷[3] 周美璐 徐文华[3] 吴颖芳[2] 方厂云[2] 彭解英[2] 

机构地区：[1]中南大学湘雅口腔医院,长沙410008 [2]中南大学湘雅医院口腔医学中心 [3]中南大学口腔医学院

出　　处：《实用口腔医学杂志》2017年第2期219-222,共4页Journal of Practical Stomatology

基　　金：教育部留学回国人员科研启动基金(编号:教外司留【2012】940);湖南省科技计划项目(编号:S2014FJ3152)

摘　　要：目的:研究wnt1在口腔黏膜下纤维性变(OSF)患者治疗前后的表达并探讨其在OSF发生发展中的作用。方法:40例OSF中期患者采用曲安奈德+丹参局部封闭治疗4周,治疗前及第5周复诊时记录VAS及张口度,取唾液及龈沟液ELISA检测wnt1表达,实时荧光定量PCR检测OSF病变颊黏膜中wnt1 mRNA表达。结果:治疗前OSF组wnt1表达[颊黏膜定量PCR(36.89±10.40)×10^(-5),唾液浓度(61.61±4.45)ng/L,龈沟液浓度(56.20±3.65)ng/L]均高于正常组[颊黏膜定量PCR(4.63±1.53)×10^(-5),唾液浓度(40.26±3.00)ng/L,龈沟液浓度(53.45±1.74)ng/L](P<0.01);OSF组经治疗后,唾液与龈沟液中wnt1表达均降低,且与OSF临床严重程度呈正相关(P<0.05)。结论:wnt1可能参与OSF的发生发展,其在唾液与龈沟液中的实时监测有望成为评价OSF诊疗的无创性检控手段之一。Objective： To observe the expression of wntl in patients with oral submucous fibrosis（OSF） before and after treatment. Methods ： 40 patients with OSF were treated with triamcinolone acetonide combined with salvia mihiorrhiza, Before and after 4 weeks treatment, pain score of VAS and mouth opening（MO） were examined, wntl protein in saliva and gingival crevicular fluid（GCF） was examined by ELISA, wntl mRNA expression in buccal mucosa tissue was examined by real-time fluorescent quantitative PCR. 20 healthy subjects were served as the controls. Results： The expression of wntl in OSF group[ buccal tissue RT-PCR （36.89 ± 10.40）×10^-5 ,saliva ELISA （61.61 ±4.45） ng/L,GCF ELISA （56.20 ±3.65） ng/L] were significantly higher than that of control group [ buccal tissue RT-PCR （4.63 ±1.53）×10^-5 ,saliva ELISA （40.26 ±3.00） ng/L, GCF ELISA （53.45 ± 1.74） ng/L） ] （P 〈 0. 01 ）. In OSF group, after treatment VAS was decreased（ P 〈 0.01 ） , M O increased （P 〈 0.01 ）） , Buecal mueosa wntl mRNA level was positively correlated with wntl protein in saliva and GCF, negativity with MO （ P 〈 0.05 ）, saliva wntl was positively correlated with VAS and GCF wntl, negitively with MO（P 〈0.05）. Conclusion： Wntl might take part in the occurrence and development of OSF. The detection of wntl in saliva and GCF might be a noninvasive method for the evaluation of OSF treatment.

关 键 词：口腔黏膜下纤维性变(OSF) WNT1 WNT/Β-CATENIN信号通路 

分 类 号：R781.5[医药卫生—口腔医学]