富血小板纤维蛋白提取液对经TNF-α刺激下人牙周膜细胞的影响  

The effects of platelet-rich fibrin extract on human periodontal ligament cells stimulated by TNF-α in vitro

作　　者：许晶晶[1] 罗子源 雷蕾[1] 

机构地区：[1]暨南大学医学院口腔医学系,广州510632 [2]深圳市人民医院口腔科

出　　处：《实用口腔医学杂志》2017年第2期223-228,共6页Journal of Practical Stomatology

基　　金：广东省自然科学基金(编号:8151063201000065)

摘　　要：目的:检测富血小板纤维蛋白提取液(PRFe)对肿瘤坏死因子α(TNF-α)刺激下的人牙周膜细胞(hPDLCs)成骨分化能力的影响。方法:组织块法分离培养hPDLCs,免疫组化鉴定其来源;Choukroun法制取PRFe;实验分为空白对照组、TNF-α(10 ng/ml)组、PRFe组和PRFe+TNF-α(10 ng/ml)组。碱性磷酸酶试剂盒检测ALP活性;茜素红染色观察细胞矿化功能;Western blotting检测Runx2、Osterix蛋白含量。结果:ALP活性检测、茜素红染色和Western blotting结果显示TNF-α组各项指标均低于空白对照组(P<0.05),PRFe组各项指标均高于空白对照组(P<0.05),PRFe+TNF-α组各项指标均高于TNF-α组(P<0.05),PRFe组各项指标均高于PRFe+TNF-α组(P<0.05)。结论:PRFe可促进经TNF-α刺激的hPDLCs成骨分化。Objective： To investigate the effects of platelet-rich fibrin extract （PRFe） on the osteogenetic differentiation and mineralization of human periodontal ligament cells （hPDLCs） stimulated by tumor necrosis factor-α （TNF-α） in vitro. Methods： hPDLCs were cultured and identified. PRFe was obtained by Choukroun＇s protocols. The cells were treated as the 4 groups： ① the blank control group, ②TNF-α （10 ng/ml）, ③ PRFe and ④PRFe ＋ TNF-α （10 ng/ml）respectively. Alkaline phosphatase activity was detected by the ALP kit. The alizarin red dye was used to observe the mineralization of the cells. Runx2 and Osterix expression was quantified by Western blotting. Results： The ALP activity, mineralization level and the expression of Runx2 and Osterin of the TNF-α group were lower that those of the control group （ P 〈 0. 05 ）. The examined indexs of PRFe group were higher than that of the control group （ P 〈 0.05 ）. The indexs of the PRFe ＋ TNF-α group were higher than that of TNF-α group （ P 〈 0.05 ） . The indexs of PRFe group were higher than that of PRFe ＋ TNF-α group（ P 〈 0.05 ）. Conclusion： PRFe may promote the osteogenetie differentiation of hPDLCs stimulated by TNF-α in vitro.

关 键 词：富血小板纤维蛋白提取液(PRFe) 肿瘤坏死因子α(TNF-α) 人牙周膜细胞(hPDLCs) 成骨分化 

分 类 号：R78[医药卫生—口腔医学]