干扰HSPC238对宫颈癌Hela细胞裸鼠成瘤的促进作用  

Promoting effect of interfering HSPC238 on cervical cancer Hela cell tumorigenesis in nude mice

作　　者：钟裕恒 黄湘[1] 吴学威[1] 梁睿[1] 

机构地区：[1]南方医科大学附属中山博爱医院产前诊断中心,广东中山528403

出　　处：《检验医学与临床》2017年第6期752-753,共2页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81101534);广东省医学科研基金资助项目(A2013875)

摘　　要：目的观察干扰HSPC238对宫颈癌Hela细胞裸鼠成瘤的影响。方法将PLL3.7-HSPC238干扰载体稳定转染Hela细胞,然后将细胞接种到BALB/c裸鼠前肢腋部皮下,观察肿瘤出现时间及体积变化。结果转染了PLL3.7-HSPC238干扰载体组的裸鼠肿瘤较对照组生长快,肿瘤的体积明显大于对照组,差异有统计学意义(P<0.05)。结论干扰HSPC238对宫颈癌Hela细胞裸鼠成瘤具有促进作用。Objective To investigate the effect of interfering HSPC238 on cervical cancer Hela cells tumorigenesis in nude mice.Methods PLL3.7-PSMA7 interfering vectors were stably transfected into Hela cells.Then the cells were subcutaneously inoculated into the anterior limb armpit of the BALB/c nude mice.The appearance time and volume change of tumor were observed.Results Compared with control group,the nude mice tumor of the transfected PLL3.7-HSPC238 interfering vector group grew more fast and the volume was bigger,the difference was statistically significant.Conclusion Interfering HSPC238 has a promoting effect on cervical cancer Hela cell tumorigenesis in nude mice.

关 键 词：HSPC238 HELA细胞 宫颈癌 裸鼠 

分 类 号：R730.5[医药卫生—肿瘤]