NG-硝基-L-精氨酸甲酯对大鼠正畸牙移动速度的影响  

Effect of NG-nitro-L-arginine methyl ester on orthodontic tooth movement in rats

作　　者：李春霞[1] 潘旭[1] 聂晶[1] 李美夏 米丛波[1] LI Chunxia PAN Xu NIE Jing LI Meixia MI Congbo(Department of Orthodontics, the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Chin)

机构地区：[1]新疆医科大学第一附属医院口腔正畸科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2017年第5期621-624,共4页Journal of Xinjiang Medical University

基　　金：新疆医科大学第一附属医院院内科研基金青年项目(2013ZRQN42)

摘　　要：目的观察诱导型一氧化氮合酶(iNOS)抑制剂NG-硝基-L-精氨酸甲酯(L-NAME)对大鼠正畸牙移动的影响,探讨一氧化氮(NO)在正畸牙牙周组织改建中的作用。方法通过正畸力牵引大鼠上颌左侧第一磨牙向近中移动,建立大鼠正畸牙移动模型。随机分为空白组、磷酸盐缓冲液(PBS)组、实验组,每组大鼠15只。空白组仅加力;PBS组于左上第一磨牙颊侧黏骨膜下局部注射无菌PBS;实验组于左上第一磨牙颊侧黏骨膜下局部注射40mg/mL的L-NAME。于加力第7、14、21天分批处死大鼠,测量牙齿移动距离,采用免疫组化法检测各组大鼠牙周组织中iNOS的表达。结果空白组和PBS组大鼠牙齿移动趋势相似,实验组牙齿移动速度减慢。加力第7、14、21天,实验组大鼠牙齿移动距离低于空白组与PBS组,差异有统计学意义(P<0.05)。各组牙齿牙周组织压力侧iNOS均有阳性表达,实验组iNOS阳性表达低于空白组和PBS组(P<0.05)。加力第7天牙周组织中iNOS阳性表达峰值最高,随后各组均呈递减趋势。结论 NG-硝基-L-精氨酸甲酯可抑制诱导型一氧化氮合酶(iNOS)的表达,减缓大鼠正畸牙移动。Objective To observe the effects of inducible nitric oxide synthase（iNOS）inhibitor NG-nitro-Larginine methyl ester（L-NAME）on tooth movement in rats and to explore the role of NO in the remodeling of orthodontic periodontal tissues.Methods 30 male SD rats were randomly divided into three groups（with 15 rats in each）.The rats of two groups received subperiosteal injectons of sterile phosphate buffer（PBS）and 40mg/ml NG-nitro-L-arginine methyl ester（L-NAME）locally once a day,respectively.The other group was just exerted with force.Rats were sacrificed at 7,14,21 days after orthodontic force to measure the distance of orthodontic tooth movement（OTM）.Hematoxylin and eosin stain and immunohistochemistry were used.Results The distance of OTM of experimental group were smallest at each time periods,comparison among groups showed there were significant differences between the experimental group and the control group（P 〈0.01）at 21 days.The expression of iNOS in periodontal tissue of experiment group was lower than that in other groups（P〈0.05）.After loading 7days the expression of iNOS were at peak,and then every group exhibited a decreasing trend.Conclusion Local inject 40 mg/ml LNAME could inhibit iNOS expression and reduce orthodontic tooth movement of rats.

关 键 词：诱导型一氧化氮合酶(iNOS) NG-硝基-L-精氨酸甲酯 正畸牙移动 平均光密度 

分 类 号：R783.5[医药卫生—口腔医学]