RB1CC1在人和小鼠口腔癌发生发展中表达的比较研究  

Expression of RB1CC1 in the Occurrence and Development of Oral Squamous Cell Carcinoma of Human and Mouse.

作　　者：陈蕾[1] 于大海[2] 闭似嫦[1] 卿海云[3] 施强[2] 孙莹[1] 

机构地区：[1]广西医科大学附属口腔医院颌面外科,广西南宁530021 [2]广西医科大学第一附属医院口腔科,广西南宁530021 [3]广西医科大学附属口腔医院病理科,广西南宁530021

出　　处：《口腔医学研究》2017年第3期240-243,共4页Journal of Oral Science Research

基　　金：国家自然基金资助项目(编号:81360407);广西自然科学基金资助项目(编号:2013GXNSFAA019182)

摘　　要：目的:比较视网膜母细胞瘤RB1-诱导卷曲蛋白1(RB1CC1)在人和小鼠正常口腔黏膜、上皮异常增生组织及口腔鳞状细胞癌中的表达,并探讨其在口腔癌发生发展过程中的作用。方法:采用免疫组化和RT-PCR检测人及小鼠在正常口腔黏膜、上皮异常增生、高分化鳞癌原发灶组织中RB1CC1蛋白及基因的表达情况。结果:RB1CC1蛋白在人上皮异常增生组、高分化鳞癌组的阳性表达高于正常组(P<0.05);RB1CC1蛋白在鼠正常组、异常增生组、高分化鳞癌组阳性表达逐渐增加,差异均有统计学意义(P<0.05)。人RB1CC1 mRNA的表达量在异常增生组与高分化鳞癌组无明显差异,正常口腔黏膜组均高于异常增生组与高分化鳞癌组(P<0.05);小鼠RB1CC1mRNA的表达量在正常口腔黏膜组与异常增生组、高分化鳞癌组差别无统计学意义。结论:RB1CC1表达在人和小鼠相似,RB1CC1可能参与了口腔鳞癌的早期癌变过程。Objective：To compare the expression of RB1-inducible coiled-coil 1（RB1CC1）in normal oral mucosa,dysplasia and oral squamous cell carcinoma（OSCC）of human and mouse model,and explore the role of RB1CC1 in the occurrence and development of OSCC.Methods：Immunohistochemical and reverse transcriptionpolymerase chain reaction method were used to detect the expression of RB1CC1 in 10cases of human normal oral mucosa,20 cases of dysplasia and 20 cases of well differentiated OSCC as well as 7cases of mouse normal oral mucosa,13 cases of dysplasia and 13 cases of OSCC,respectively.Results：In human,the positive expression rates of RB1CC1 in dysplasia and well differentiated squamous cell carcinoma group were higher than that in normal oral mucosa group（P〈0.05）.In mouse,the positive expression rate of RB1CC1 gradually increased from normal to carcinoma group（P〈0.05）.By contrast,in human,the gene expression of RB1CC1 in normal oral mucosa was higher than dysplasia and OSCC group（P〈0.05）,but not significantly different in dysplasia and well differentiated OSCC group.Conclusion：The expression of RB1CC1 was similar in the same pathological stages of between human and mouse,and may play an important role in early carcinogenesis of OSCC.

关 键 词：视网膜母细胞瘤 RB1-诱导卷曲蛋白1(RB1CC1) 口腔鳞状细胞癌 人 小鼠 

分 类 号：R739.8[医药卫生—肿瘤]