结肠癌患者血清中microRNA-183及胸苷激酶1的表达及意义  

The expression and significance of serum microRNA-183 and TK1 in patients with colorectal cancer

作　　者：王金亮[1] 孟祥朝[2] 张自立[2] 李琦[2] 

机构地区：[1]天津医科大学三中心临床学院,300170 [2]天津市第三中心医院

出　　处：《天津医药》2017年第1期72-75,共4页Tianjin Medical Journal

摘　　要：目的检测结肠癌患者血清中mi RNA-183及胸苷激酶1(TK1)的表达并探讨其临床意义。方法收集结肠癌患者及健康体检者血清标本各52例,荧光定量PCR检测mi RNA-183表达,免疫印迹增强化学发光法检测TK1的表达,分析两者之间的相关性及与临床病理的关系。结果与正常对照组相比,结肠癌患者血清mi R-183的表达相对升高(P<0.01);中低分化腺癌者血清mi R-183的表达量高于高分化腺癌(P<0.01);Ⅲ~Ⅳ期结肠癌者血清中mi R-183的表达量高于Ⅰ~Ⅱ期(P<0.01),且在有淋巴结转移的患者中表达较高(P<0.01);受试者工作特征(ROC)曲线显示,以相对表达量1.15为临界点,血清mi R-183可能具有诊断结肠癌的价值,其敏感度为78.8%,特异度为67.3%,阳性预测值为78.9%,阴性预测值为70.2%。结肠癌患者血清中TK1的表达也较正常对照组升高,中低分化腺癌高于高分化腺癌(P<0.05),Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期(P<0.01)。血清中mi R-183与TK1的表达呈正相关(rs=0.692,P<0.01)。结论血清中mi R-183及TK1可能是早期结肠癌的诊断标志物,并可用于判断肿瘤的恶性程度及预后。Objective To detect the expression and significance of serum miRNA- 183 and TK1 in patients withcolorectal cancer, and the mechanism thereof. Methods Fifty-two serum samples of colorectal cancer patients and pairedhealth serum samples were collected. The expression of miRNA-183 was detected by real-time quantitative PCR, and TK1 was detected by Western blot enhanced chemiluminescence assay. The correlation between miRNA-183 and TK1 and theirrelations with the clinicpathologic characteristics were analyzed. Results The serum miRNA- 183 expression wassignificantly higher in colorectal cancer group than that in normal control group（P 〈 0.01）. The expression of serum miRNA-183 was significantly higher in stage Ⅲ-Ⅳ group than that in stage Ⅰ-Ⅱ group（P 〈 0.01）. There was more significantincrease in serum miRNA- 183 in lymphatic metastasis group than that without lymphatic metastasis group（P 〈 0.01）.Receiver operating characteristic（ROC） curve showed that of there was a diagnostic value for serum miRNA- 183 incolorectal cancer, with an optimal value of 1.15. The diagnostic sensitivity and specificity were 78.8% and 67.3%, and thepositive predictive value and negative predictive value were 78.9% and 70.2%. The serum TK1 expression was also higher incolorectal cancer group compared with that of normal control group（P 〈 0.01）. And the TK1 expression was also higher instage Ⅲ-Ⅳ group than that in stage Ⅰ-Ⅱ group（P 〈 0.01）. Furthermore, miRNA-183 expression was positively correlatedwith TK1 expression（rs=0.692, P 〈 0.01）. Conclusion The serum expression levels of mi R-183 and TK1 may act as tumormarkers for early colorectal cancer diagnosis, and also can be used to predict the malignant degree and prognosis ofcolorectal cancer.

关 键 词：结肠肿瘤 微RNAS 胸苷激酶 血清 结肠癌 miRNA-183 

分 类 号：R735.35[医药卫生—肿瘤]