便携式体外生命支持系统离体和在体实验研究  

Portable extracorporeal life support system in vitro and in vivo experiment

作　　者：刘宇[1] 周圆圆[2] 尹宗涛[1] 李洪谊[2] 王辉山[1] 

机构地区：[1]沈阳军区总医院心血管外科,沈阳110016 [2]中国科学院沈阳自动化研究所,沈阳110167

出　　处：《中国体外循环杂志》2017年第1期38-41,共4页Chinese Journal of Extracorporeal Circulation

基　　金：2013年军队装备研制项目重大项目(ASY135001);2014年辽宁省建设产业技术创新体系示范工程医疗器械临床研究平台(2014305006)

摘　　要：目的初步考察便携式体外生命支持系统的离体和在体性能。方法离体实验检测便携式体外生命支持系统的稳定性;在体实验中12只雌性巴马香猪随机分为实验组和对照组,每组6只。分别应用便携式体外生命支持系统及Maquet公司Rotaflow系统进行体外循环,4.0 L/min运转6 h,于转流前、转流中每2 h及转流后取静脉血检测游离血红蛋白含量评价装置溶血性能并检测血小板激活率。结果离体实验证实便携式体外生命支持系统稳定性优于或等于同类Maquet公司Rotaflow系统;在体实验提示4.0 L/min流量运行6 h,血浆游离血红蛋白含量均在50 g/L以下,血小板激活均小于8%。结论便携式体外生命支持系统离体和在体性能稳定、可靠。Objective To explore the performance of a portable extracorporeal life support（ PELS） system in vitro and in vivo.Methods In vitro,PELS system was tested for stable assessment; in vivo,12 female swine were randomized into experiment group（ n= 6） and control group（ n = 6）. Cardiopulmonary bypass（ CPB） was repectively established with PELS and Rota Flow with 4 L/min of flow for 6 h. The plasma free hemoglobin and platelet activation were evaluated before initial of CPB,every 2 hs in CPB and after terminated CPB. Results In vitro,experiments confirmed that the stability of PELS system was better than or equal to Maquet company Rotaflow system; in vivo,the plasma free hemoglobin was lower than 20 g/L and the platelet activation was lower than 10% after 6 h performance of PELS system. Conclusion The portable extracorporeal life support system conducts the reliable performance in vitro and in vivo.

关 键 词：便携 体外生命支持 动物实验 体外实验 

分 类 号：R654.1[医药卫生—外科学]