TERT基因转染BMSC血管性痴呆大鼠记忆功能及海马CA1区突触可塑性的影响  

Effect of TERT gene transfected BMSC on memory function and hippocampal CA1 region synaptic plasticity in vascular dementia rat

作　　者：段金旗[1] 马丽琼[2] 刘远林[1] 任炜[1] 陈媛媛[1] 李春岩[3] 

机构地区：[1]张家口学院医学院,河北张家口075000 [2]解放军251医院重症医学科,河北张家口075000 [3]河北医科大学第二医院神经内科,石家庄050000

出　　处：《重庆医学》2017年第10期1300-1303,1307,共5页Chongqing medicine

基　　金：国家自然科学基金资助项目(81171210)

摘　　要：目的探讨端粒酶逆转录酶(TERT)基因转染骨髓基质干细胞(BMSC)对血管性痴呆大鼠记忆功能及海马CA1区突触可塑性的影响。方法 60只大鼠随机分为阴性对照组(A组)、模型组(B组)、常规BMSC组(C组)和转染BMSC组(D组);采用Morris迷宫测试、RT-PCR和蛋白免疫印迹法检测各组大鼠相关指标。结果 C组、D组逃避潜伏期显著长于B组;D组逃避潜伏期显著长于B组。与A组比较,B组、C组、D组脑源性神经营养因子(BDNF)mRNA、TERT mRNA、突触前区突触素(SYP)mRNA及蛋白表达均显著降低,A组大鼠突触间隙排列清晰,SYN阳性细胞数较多;D组突触间隙更为清晰,SYN阳性细胞数量和突出间隙排列结构都接近于A组。结论 TERT转染BMSC对血管性痴呆大鼠的治疗作用明显,其作用机制与促进BDNF、TrkB表达、改善突触的可塑性有关。Objective To explore the effect of telomerase reverse transcriptase（TERT） gene transfected bone marrow stem cell（BMSC）on the memory function and hippocampal CA1 region synaptic plasticity in vascular dementia rat. Methods A total of 60 rats were randomly divided into the negative control group（group A）,model group（group B）, conventional BMSC group（group C） and transfected BMSC group（group D）. The related indicators in each group were detected by using the Morris maze test,RT- PCR and Western blot respectively. Results The escape latency period in the group C and group D was significantly longer than that in the group B,which in the group D was significantly longer than that in the group C. Compared with the group A, the expres- sions of brain-derived neurotroPhic factor（BDNF）mRNA, TERT mRNA, SYP mRNA and protein in the group B, group C and group D were significantly decreased. The synaptic cleft arrange in group A was clear with more SYN positive cells. The synaptic cleft in the group D was clearer,and the number of SYN positive cells was close to that iri group A. Conclusion TERT transfected BMSC has obvious therapeutic effect on vascular dementia rats and its mechanism may be related to the promotion of BDNF,TrkB expression and the improvement of synaptic plasticity.

关 键 词：端粒酶逆转录酶 骨髓基质干细胞 痴呆 血管性 记忆功能 突触 可塑性 

分 类 号：R749.13[医药卫生—神经病学与精神病学]