肿瘤生物标志物的超灵敏检测研究进展  

Advance in development of methods for sensitive detection of tumor-related biomarkers

作　　者：许爱君[1] 王子月[1] 张春阳[1] XU AiJun WANG ZiYue ZHANG ChunYang(College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China)

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《科学通报》2017年第9期859-870,共12页Chinese Science Bulletin

基　　金：国家杰出青年科学基金(21325523);科技部重点领域创新团队计划(2015RA4024)资助

摘　　要：肿瘤是严重威胁人类健康的高发病率和高死亡率疾病.肿瘤生物标志物是反映肿瘤的发生和发展、监测肿瘤对治疗反应的一类物质.肿瘤生物标志物的超灵敏检测有助于肿瘤的早期诊断和临床治疗.近年来,肿瘤生物标志物的检测方法研究迅猛发展,成为国内外研究的热点.本文对最新发展的肿瘤生物标志物检测方法进行了全面综述,着重介绍了比色法、质谱法、荧光分析法、化学发光、表面增强拉曼散射、电化学分析法和单分子检测,并对肿瘤生物标志物检测方法的发展方向做了展望.Tumors are formed by the unlimited proliferation of normal cells in living body and pose a serious threat to human health with high morbidity and mortality. Tumor-related biomarkers are a class of substances resulting from the tumor cell formation, release, or the body＇s response to tumor cells, which are generated and increase during tumor development and proliferation. Tumor-related biomarkers may be divided into two categories： one is inside cancer cells （e.g., microRNA （miRNA）, telomerase and transcription factor） and the other is on the cancer cell membranes （e.g., epithelial membrane antigen （EMA） and carcino-embryonic antigen （CEA））. Tumor-related biomarkers may reflect tumorigenesis, progression, and the responses to drug treatment. Consequently, the sensitive detection of tumor-related biomarkers is essential to early clinical diagnosis and therapy. Polymerase chain reaction （PCR） and immunohistochemistry technique have frequently been used to detect tumor-related biomarkers, but they are usually laborious and time-consuming. In recent years, sensitive detection of tumor-related biomarkers has become a hot area, with great progress having been made. A variety of methods including colorimetric, mass spectrometry, fluorescent, chemiluminescent, surface enhanced Raman scattering （SERS）, electrochemical and single-molecule detection approaches have been developed for sensitive detection of tumor-related biomarker. Colorimetric assay has significant advantages of visualization, simple operation, high sensitivity, and no need for delicate instruments. The advantages of inductively coupled plasma mass spectrometric （ICPMS） include low detection limits, low matrix effects, large dynamic ranges and high spectral resolution for elements and isotopes. Fluorescent method allows for the homogeneous assay of biomarkers in solution with significant advantages of simplicity, low cost, high sensitivity, and safety without the requirement of radioisotopes. Chemiluminescent assay does no

关 键 词：肿瘤生物标志物 癌症 肿瘤细胞 超灵敏检测 分析方法 发展方向 

分 类 号：R730.4[医药卫生—肿瘤]