颞下颌关节紊乱患者关节液中MMP-3和MMP-9表达的临床意义  

Clinical Significance of Expression of MMP-3 and MMP-9 in Joint Fluid of Patients with Temporomandibular Disorder

作　　者：刘建林[1] 王岩松[1] 刘美洁[2] 周思影 陈曦[2] LIU Jian-lin WANG Yan-song LIU Mei-jie ZHOU Si-ying CHEN Xi(Department of Stomatology, Shenzhen Hospital of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518034, China Department of Stomatology, The First Affiliated Hospital of Xi＇an Jiao Tong University, Xi＇an, Shaanxi, 710061, China)

机构地区：[1]广州中医药大学深圳医院口腔科,广东深圳518034 [2]西安交通大学第一附属医院口腔科,陕西西安710061

出　　处：《现代生物医学进展》2017年第6期1117-1119,共3页Progress in Modern Biomedicine

基　　金：深圳市科技研发基金项目(JCYJ20120827172450676)

摘　　要：目的:研究颞下颌关节紊乱(TMD)患者关节液中基质金属蛋白酶-3(MMP-3)和基质金属蛋白酶-9(MMP-9)水平变化及临床意义,为临床诊疗提供依据。方法:选取2013年4月到2016年4月我院收治的TMD患者60例,根据患者病变程度分为炎性疾病组(n=21例)、结构紊乱组(n=18例)、骨关节病组(n=21例),另选取同期健康志愿者30例为对照组采集研究者的关节液标本并应用双抗体夹心酶联免疫法检测关节液标本中MMP-3和MMP-9的水平。结果:骨关节病组MMP-3和MMP-9水平显著高于结构紊乱组、炎性疾病组和对照组,结构紊乱组、炎性疾病组显著高于对照组,比较差异具有统计学意义(P<0.05),结构紊乱组与炎性疾病组比较差异无统计学意义(P>0.05)。结论:关节液中MMP-3和MMP-9水平随着TMD病变程度增加而明显升高,能在一定程度上反应颞下颌关节病严重程度。Objective：To study the level change and clinical significance of matrix metalloproteinase-3（MMP-3） and matrix metalloproteinase-9（MMP-9） in the joint fluid of patients with temporomandibular disorder,to provide basis for clinical diagnosis and treatment.Methods：60 cases of temporomandibular disorders who were treated in our hospital from April 2013 to April 2016 were selected,they were divided into inflammatory disease group（n=21）,structural disorder group（n=18）,bone and joint disease group（n=21cases） according to the degree of patient’s disease,during the same period 30 cases of healthy volunteers were selected as control group,collected joint fluid samples of the researchers.The levels of MMP-3 and MMP-9 were detected by double antibody sandwich enzyme linked immunosorbent assay.Results：The levels of MMP-3 and MMP-9 in the bone and joint disease group was significantly higher than that of structure disorder group,inflammatory diseases group and control group,which of structural disorder group and inflammatory diseases group were significantly higher than those in the control group,the differences were statistically significant（P〈0.05）,there was no significant difference between the structural disorder group and inflammatory diseases group（P〉0.05）.Conclusion：The level of MMP-3 and MMP-9 in joint fluid increases significantly with the degree of lesion,which can reflect the severity of temporomandibular disease.

关 键 词：颞下颌关节紊乱 基质金属蛋白酶-3 基质金属蛋白酶-9 临床 意义 

分 类 号：R782.6[医药卫生—口腔医学]