异氟醚对人乳腺癌MDA-MB-231细胞的影响及相关机制探讨  

Effects of isoflurane on human breast cancer MDA-MB-231 cells and discussion on its relevant mechanism

作　　者：冯娟[1] 吴满武[2] 

机构地区：[1]绍兴市妇幼保健院麻醉科,浙江绍兴312000 [2]绍兴市妇幼保健院遗传室,浙江绍兴312000

出　　处：《中国现代医生》2017年第5期8-11,共4页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2015KYB405)

摘　　要：目的探讨异氟醚对人乳腺癌MDA-MB-231细胞增殖凋亡的影响机制。方法采用MTT法观察异氟醚作用24 h对MDA-MB-231细胞(密度5×10~5/孔)增殖能力的影响;采用Western blot法检测磷酸化Akt(蛋白激酶B,protein kinase B),Bcl-xl(B细胞淋巴瘤/白血病-xl基因,B-cell lymphoma-extra large)、Bad(人促凋亡蛋白,Human apoptosis protein)的蛋白表达情况,以探究其对细胞凋亡的调控机制。结果(1)异氟酿对人乳腺癌MDAM B-231细胞增殖的抑制作用,随着时间的延长逐渐增强,与对照组相比,差异均有显著统计学意义(P<0.05)。结果可见异氟醚对人乳腺癌MDA-MB-231细胞増殖起到抑制作用,且具有明显的量效与时效关系;(2)各组磷酸化Akt的表达变化差异有统计学意义(P<0.05)。与对照组相比、异氟醚组磷酸化Akt在麻醉24h降低63.1%(P<0.05),48 h及72 h均呈回升趋势(P<0.05);(3)实验组咅时点Bad表达的变化差异没有统计学意义(P>0.05)。但各时点Bcl-xl表达的变化有显著差异(P<0.05),7+2.h Bcl-xl表达较对照组下降82.2%(P<0.05)。结论异氟醚可抑制人乳腺癌MDA-MB-231细胞增殖,并可通过Akt/Bd细胞通路诱导其细胞凋亡。Objective To investigate the effect of isoflurane on the proliferation and apoptosis of human breast cancer cell line MDA-MB-231. Methods MTr was applied to observe the effect of isoflurane on the proliferation ability of MDA-MB-231 cells（density 5×10^5/well）; Western blot was applied to detect the expression of phosphorylated Akt （pro- tein kinase B）, Bcl-xl （B cell lymphoma-extra large）, Bad （human proapoptotic protein）, in order to explore its regulat- ing mechanism of apoptosis. Results （1）The inhibitory effect of isoflurane on the proliferation of human breast cancer MDA-MB-231 cells was gradually increased with the prolongation of time. Compared with the control group, there was statistical significance in each group（P〈0.05）. The results showed that isoflurane could inhibit the proliferation of human breast cancer MDA-MB-231 cells, and it had significant dose-effect and time-effect relationship; （2）The differences of changes of expression of phosphorylated Akt in each group were statistically significant（P〈0.05）. Compared with the control group, phosphorylated Akt in isoflurane group was decreased by 63.1%（P〈0.05） at 24 h of anesthesia and was increased at 48 h and 72 h（P〈0.05）; （3） There was no statistically significant difference in the changes of the expression of Bad in the experimental group at each time point（P〉0.05）. However, the changes of the expression of Bcl-xl at all time points were significantly different（P〈0.05）, and the expression of 72 h Bcl-xl was reduced by 82.2% compared with the control group（P〈0.05）. Conclusion Isoflurane can inhibit the proliferation of human breast cancer MDA-MB- 231 cells and induce apoptosis by Akt/Bad cell pathway.

关 键 词：异氟醚 乳腺癌 MDA-MB-231细胞 增殖 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]