上皮-间质转化在口腔黏膜下纤维性变中的作用  

Epithelial-Memsenchymal Transitions Effects on Oral Submucoous Fibrous

作　　者：赵国强[1] 王佳婷 郑灵娥 张鹏[1] 王舒仪 李江[4] 施更生[1] 

机构地区：[1]温州医科大学附属台州医院口腔科,317000 [2]恩泽医院内科,台州317000 [3]温州医科大学附属台州医院口眼中心,317000 [4]中南大学湘雅医院口腔科,长沙410000

出　　处：《医学研究杂志》2017年第3期111-115,共5页Journal of Medical Research

摘　　要：目的研究上皮-间质转化(epithelial-to-mesenchymal transitions,EMT)中主要相关因素上皮细胞钙黏蛋白(Ecadherin,E-cad)和Slug在口腔黏膜下纤维性变(oral submucous fibrous,OSF)组织中的作用研究。方法采用荧光定量PCR法(Q-PCR)及免疫组织化学法检测41例OSF患者和19例正常口腔黏膜组织切片中E-cad和Slug的表达,分析E-cad与Slug的表达在OSF和正常口腔黏膜组织中的异常表达及E-cad与Slug的异常表达的相互作用关系。结果免疫组化结果显示在OSF组织中E-cad和Slug的阳性表达率分别为70.73%(29/41)和41.46%(17/41),同时发现OSF组织中E-cad的表达与Slug的表达为负相关(r=-0.656,P<0.05)。荧光定量PCR结果显示OSF组织中Slug的表达高于正常口腔黏膜组织(P<0.05),而OSF组织中E-cad的表达低于正常口腔黏膜组织(P<0.05)。结论 EMT在OSF形成及恶变中可能起重要的作用,其主要相关基因E-cad与Slug的检测对预防OSF恶变成口腔癌提供重要参考指标。Objective To study the effects of slug and E - cadherin （E - cad）, the mainly relative element in the Epithelial - Memsenchymal Transitions, on the oral submucous fibrous（ OSF）. Methods We detected the expressions of E - cad and Slug of 41 OSF patients and 19 normal oral mucosal biopsies by adopting the fluorescene quantitative polymerase chain reaction assay （Q -PCR） and im- munohistochemical method ; analyzed the expressions of E - cad and slug in OSF patients and the abnormal expressions of E - cad and slug in normal oral mucosa, and analyzed the relationship between the abnormal expressions of E - cad and slug and interreaction. Results Immunohistochemical analysis showed that the positive expression rates of E - cad and slug in OSF musoca were respectively 70.73% （29/ 41 ） and 41.46% （ 17/41 ） , and it also showed a negative relation between the expression of E - cad and the expression of slug in OSF musoca. The Q - PCR analysis showed that the expression of slug in OSF musoca was higher than in normal oral musoca （ P 〈 0. 05 ） , but the expression of E - cad in OSF musoca is lower than in normal oral musoca （P 〈 0.05）. Conclusion EMT probably plays an important role in the formation and canceration of OSF, and the detection of mainly relative genes, E - cad and slug can provide important reference index for preventing OSF from cancerating to oral cancinoma.

关 键 词：E—cadherin SLUG EMT OSF Q—PCR 免疫组织化学 

分 类 号：R78[医药卫生—口腔医学]