lincRNA-p21抑制肾癌细胞增殖作用  

Efffect of LincRNA-p21 on Proliferation of Renal Cell Carcinoma

作　　者：潘珠玛 黄文瀚[2] 李达学[3] 

机构地区：[1]重庆市第七人民医院内分泌肾病科,400054 [2]重庆医科大学附属第二医院风湿免疫科 [3]重庆医科大学附属第一医院内分泌乳腺外科

出　　处：《医学研究杂志》2017年第3期149-152,共4页Journal of Medical Research

摘　　要：目的探讨lincRNA-p21对肾癌细胞增殖作用的影响。方法收集17例重庆市第七人民医院内分泌肾病科肾癌患者的组织样本,利用RT-PCR检测肾癌及其癌旁组织中lincRNA-p21的表达水平及人肾癌细胞系786-O、人胚肾细胞系HEK-293细胞中lincRNA-p21的表达水平,用siRNA降低lincRNA-p21的表达后观察其对细胞增殖、凋亡情况的影响以及对Bax、Noxa、Puma等p53信号通路中癌症相关基因表达水平的影响。结果在肾癌患者的癌灶组织中,lincRNA-p21的表达水平明显高于癌旁组织(P<0.05),786-O较HEK-293细胞lincRNA-p21 mRNA表达水平显著升高(P<0.05);用siRNA降低lincRNA-p21的表达后发现786-O细胞增殖增强,凋亡减弱,并且Bax、Noxa、Puma等癌症相关基因表达水平也被抑制(P<0.05)。结论 lincRNA-p21可能通过参与p53信号通路的转导,对肾癌细胞的增殖起到抑制作用,提示lincRNA-p21可作为分子靶点用于肾癌的靶向治疗。Objective To study the effect of lincRNA - p21 on the proliferation of renal cell carcinoma. Methods The expression levels of lincRNA - p21in renal cell carcinoma tissues and their corresponding para - cancerous tissues from 17 patients in department of nephrology,Hua Xi hospital, was detected by RT - PCR. In addition, the expression of lincRNA - p21 was analyzed in human clear cell RCC cell line 786 - O and human embryonic kidney cell line HEK - 293. The cell proliferation, apoptosis and the expression of Bax, Noxa,Puma that participated in p53 signaling pathway were detected after lincRNA - p21 was downregulated with siRNA. Results The expression levels of lincRNA - p2lwas higher in renal cell carcinoma tissues and 786 - O ceils than para - cancerous tissues and HEK - 293 cells respectively（P 〈 0.05 ）. The ability of proliferation was significantly increased after lincRNA - p21 siRNA treatment in 786 - O cells, whereas the cell apoptosis was decreased（ P 〈 0.05 ）. The mRNA expressions of Bax, Noxa, Puma were also dramatically decreased after lincRNA - p21 was downregulated（P 〈 0.05）. Conclusion LincRNA - p21 inhibits the cell proliferation of 786 - O cells through participation in p53 signal transduction pathways. These data suggested that lincRNA - p21 maybe could be used as molecular targets for human RCC treatment.

关 键 词：lincRNA—p21 肾癌增殖 P53 

分 类 号：R737.11[医药卫生—肿瘤]