大鼠心脏表面血流动态检测及其在心肌梗死模型实时鉴定中的应用  

Dynamic detection of surface blood flow in rat heart and its application in real time identification of myocardial infarction model

作　　者：雷倩 陈灿 吴小玲 陈文江 易焘 马睦棣 何原 税晓容 黄石安 雷桅 

机构地区：[1]广东医科大学附属医院心血管内科中心,广东省湛江市524001

出　　处：《中华医学杂志》2017年第13期1019-1023,共5页National Medical Journal of China

基　　金：基金项目：国家自然科学基金项目（81270262,81300035）;广东省协同创新与平台环境建设专项（2015A050502049）;广东省科技发展专项（2016A020214017）,广东省医学科研基金（A2015286）

摘　　要：目的建立监测大鼠心脏表面血流动态的方法，并明确其心肌梗死（简称心梗）程度与心脏表面血流灌注量的关系，从而为大鼠心梗模型的鉴定提供一种新指标。 方法将大鼠分为对照组（n＝23）和模型组（n＝107），采用激光多普勒血流灌注成像仪对大鼠术前和术后心脏分别进行扫描和数据分析，获得心脏表面血流变化率。用NBT法检测模型组大鼠的心肌梗死范围，根据梗死范围将该组分成轻度心梗、中度心梗和重度心梗3个亚组，并分析其与心脏表面血流变化率的关系。 结果模型组大鼠的心肌梗死范围与心脏表面血流变化率呈高度相关（r＝0.849 6，P〈0.000 1），就亚组而言，轻度心梗亚组无显著相关性（r＝－0.133 6，P〉0.05），中度心梗亚组的相关性较强（r＝0.721 7，P〈0.000 1），而重度心梗亚组表现出最高相关性（r＝0.910 2，P〈0.000 1）。 结论大鼠心脏表面血流动态与心肌梗死程度关系密切，其血流灌注量变化可作为心梗模型建立和鉴定的有效参考。ObjectiveTo establish a method for monitoring the surface blood flow in the heart of rats, and to clarify the relationship between the degree of myocardial infarction and the blood perfusion on the surface of the heart, so as to provide a new indicator for the identification of rat myocardial infarction model. MethodsThe rats were divided into control group （n=23） and model group （n=107）, the rat hearts were scanned by the laser doppler perfusion imager before and after operation respectively, and the data was analyzed to acquire the rate of surface blood flow change of the heart. Myocardial infarction size of model group was detected by NBT. Model group were divided into three subgroups of mild myocardial infarction, moderate myocardial infarction and severe myocardial infarction according to the myocardial infarction size, and an analysis was made on the correlativity between rate of surface blood flow change of the heart and myocardial infarction size. ResultsMyocardial infarction size was highly correlated to the rate of surface blood flow change of the heart in model group （r=0.849 6, P〈0.000 1）. There was no significant correlation between infarction size and heart blood flow in the mild myocardial infarction subgroup （r=-0.133 6, P〉0.05）, while the correlation in moderate myocardial infarction was significant （r=0.721 7, P〈0.000 1）, and the highest correlation was shown in severe myocardial infarction subgroup （r=0.910 2, P〈0.000 1）. ConclusionThe heart surface blood flow has a close relationship with the myocardial infarction size in rat, so the change of heart blood perfusion can beused as an effective reference to establish and identify rat myocardial infarction model.

关 键 词：疾病模型 动物 心脏 结扎术 心肌缺血 局部血流 

分 类 号：R-332[医药卫生] R542.22