巨噬细胞移动抑制因子在非小细胞肺癌患者血清及组织中的表达及意义  

Expression and Clinical Significance of Macrophage Migration Inhibitory Factor in the Serum and Lung Tissues in Patients with Non-Small Cell Lung Cancer

作　　者：张洁[1] 廖慧芳[2] 郭禹标[3] 

机构地区：[1]广州医科大学附属第一医院重症医学科,广东广州510080 [2]中山大学附属第一医院检验科,广东广州510080 [3]中山大学附属第一医院呼吸内科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2017年第2期243-249,共7页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(10151008901000242)

摘　　要：【目的】检测非小细胞肺癌(NSCLC)患者血清及肺癌组织中巨噬细胞移动抑制因子(MIF)的表达水平,探讨MIF在NSCLC患者中的表达及其临床意义。【方法】NSCLC组患者66例,肺部良性病变组患者22例,健康体检者30例,用酶联免疫吸附法(ELISA)检测各组血清中MIF的浓度水平;用免疫组织化学法(IHC)检测两组患者肺组织中MIF的表达水平。应用SPSS17.0统计学软件进行分析。【结果】(1)NSCLC患者血清MIF浓度显著高于健康人(14.79 ng/mL vs 10.69 ng/mL,P=0.001),略高于肺良性病变组但无统计学差异(14.79 ng/mL vs 13.68 ng/mL,P=0.580)。NSCLC患者血清中MIF的水平与肿瘤的分化程度、临床分期及患者的性别、吸烟状态有关。(2)免疫组化结果显示,肺癌组织中MIF的高表达率显著高于肺良性病变组织(30.3%vs 4.5%,P=0.014),且MIF的表达程度与肿瘤的病理类型、临床分期相关。(3)相关性分析显示,NSCLC患者血清MIF水平与肺癌组织的MIF高表达率呈正相关(P<0.05)。【结论】MIF在NSCLC患者中的表达与肺癌的临床病理特征有一定的关系,对NSCLC的诊断及临床评估有一定的价值。【Objective】To investigate the expression and the clinical significance of macrophage migration inhibitory factor（MIF）in the serum and lung tissues of the patients with non-small cell lung cancer（NSCLC）.【Methods】Eighty-eight eligible inpatients were confirmed by pathology of lung tissues,including 66 patients with NSCLC and 22 patients with benign lung lesions.ELISA was performed to measure serum concentration of MIF of these patients,which was compared with 30 healthy individuals.Meanwhile,immunohistochemistry（IHC）was performed to examine the expression of MIF in the lung tissues of the two groups.MIF expression level was compared between two groups and among different subgroups of NSCLC.The correlationship between serum MIF level and high expression rate in lung tissues was also analyzed.All the data were analyzed by SPSS17.0.【Results】The serum concentration of MIF in NSCLC group was significantly higher than that in healthy control（14.79 ng/mL vs 10.69 ng/mL,P=0.001）,and was slightly higher but not significantly different from benign lung lesions group（14.79 ng/mL vs 13.68 ng/mL,P=0.580）.The comparison among subgroups of NSCLC showed that the serum MIF level was not only correlated with the histological grade and clinical stage of the cancer,but also correlated with the gender and smoking history of the host.Immunohistochemistry showed that the MIF high expression rate in the lung tissues of NSCLC was markedly higher than that of benign lung lesions group（30.3% vs 4.5%,P=0.014）.Among the subgroups of NSCLC,IHC showed MIF high expression rate was correlated with the histopathologic types andclinical stage of the cancer.Simultaneously,the serum MIF level showed a positive correlation with MIF expression rate in the lungtissues in all patients and NSCLC group（P0.05）.【Conclusion】MIF was strongly related to the clinicopathological characteristicsof NSCLC.It could be helpful for the diagnosis and clinical evaluation of NSCLC.

关 键 词：巨噬细胞移动抑制因子 非小细胞肺癌 生物学标志物 

分 类 号：R734.2[医药卫生—肿瘤]