缺氧缺血性脑病新生小鼠脑组织pCaMKⅡα表达和空间学习记忆的变化  

Changes of pCaMKⅡα expression in brain and spacial learning and memory in neonatal hypoxic-ischemia encephalopathy mice

作　　者：肖爱娇[1] 汪建民[1] 刘结民 欧阳昕[2] Xiao Aijiao Wang Jianmin Liu Jiemin Ouyang Xin(School of Basic Medical Science, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China School of Moxibustion Science,Jiangxi University of Traditional Chinese Medicine ,Nanchang 330004, China)

机构地区：[1]江西中医药大学基础医学院,南昌330004 [2]江西中医药大学灸学院,南昌330004

出　　处：《中华行为医学与脑科学杂志》2017年第3期193-197,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81060305,81660819）;江西省自然科学基金项目（20151BAB205068,20161BBH80053）;江西省卫生计生委中医药科研计划重点项目（20142003）;江西中医药大学课题（2014ZR018,2015jzzdxk024）

摘　　要：目的观察缺氧缺血性脑病新生小鼠脑组织神经元核抗原（NeuN）、磷酸化钙／钙调蛋白依赖性蛋白激酶Ⅱα（PCaMKⅡα）表达和空间学习记忆的变化。方法7d新生小鼠，随机分为两组：假手术组（n=19）与模型组（n=23）。采用右侧颈总动脉结扎，并给予8％低氧100min制备新生小鼠缺氧缺血性脑病模型；DAPI染色观察脑组织病理变化；荧光免疫组织化学法检测脑组织NeuN和pCaMKⅡα的表达；Morris水迷宫测试小鼠的空间学习记忆力。结果假手术组小鼠脑组织细胞排列致密整齐，NeuN阳性细胞数[（106．50±20．07）个]和pCaMKⅡα阳性细胞数[（87．17±16．55）个]较多，逃避潜伏期短；与假手术组相比，模型组小鼠患侧脑组织可见大量细胞坏死脱落、细胞间隙变大，NeuN阳性细胞数[（19．17±3．60）个]和pCaMKⅡα[阳性细胞数[（13．33±3．62）个]明显减少（P〈0．01），逃避潜伏期延长（P〈0．01）。结论缺氧缺血性脑病模型小鼠空间学习记忆力差，可能与脑组织神经元上pCaMKⅡα表达减少有关。Objective To observe the expressions of NeuN and pCaMK Ⅱα in brain and test the spacial learning and memory in neonatal hypoxic-ischemia encephalopathy （HIE） model mice. Methods 7d ICR mice were randomly divided into sham group（ n= 19） and model group（n=23）. HIE model was induced by right common carotid artery ligation followed by 8% oxygen hypoxia for 100 min. DAPI staining was used to examine brain pathological change,immunofluorcscent staining was used to examine the expression of NeuN and pCaMK Ⅱα in the ipsilateral brain, and Morris water maze was used to test the spacial learning and memory. Results Mice in sham group showed that brain cells were arranged in a dense and orderly manner, the number of NeuN-positive cells and pCaMKⅡα-positive cells were （ 106.50±20.07）, （87.17±16.55） respectively in the brain,and the escape latency was short. Compared with mice in sham group, mice in model group showed more cells loss,less NeuN-positive cells（19.17±3.60） and less pCaMKⅡα-positive cells（13.33±3.62） in the ipsilateral hemisphere,and longer escape latency（P〈0.01 ）. Conclusion The spacial learning and memory are impaired in hypoxia ischemia, which may be related to the de- creasing expression of pCaMK Ⅱα in neurons in ipsilateral brain.

关 键 词：缺氧缺血性脑病 磷酸化钙/钙调蛋白依赖性蛋白激酶Ⅱα 神经元核抗原 空 间学习记忆 

分 类 号：R742[医药卫生—神经病学与精神病学]